PXD039401 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of the E3 ligase(s) recruited by 955 to degrade CDK9 |
Description | PROTACs induce the degradation of target proteins by hijacking an E3 ligase. However, it is usually difficult to directly identify the E3 ligase recruited by a new E3 ligase ligand in a PROTAC using the co-immunoprecipitation method because the formation of the complex is transient and very dynamic. TurboID assay is a powerful proximity labeling method that can sensitively detect weak and transient protein-protein interactions by biotinylating proteins that interact with a bait protein fused with an engineered biotin ligase. Here, we adapted TurboID technology to identify and characterize the specific E3 ligase(s) recruited by 955, a potent piperlongumine (PL)-SNS-032 conjugated CDK9 PROTAC, to mediate CDK9 degradation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-24 |
AnnouncementXML | Submission_2024-05-24_01:04:06.802.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dongwen Lyu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-13 06:04:08 | ID requested | |
⏵ 1 | 2024-05-24 01:04:07 | announced | |
Publication List
Pei J, Xiao Y, Liu X, Hu W, Sobh A, Yuan Y, Zhou S, Hua N, Mackintosh SG, Zhang X, Basso KB, Kamat M, Yang Q, Licht JD, Zheng G, Zhou D, Lv D, Piperlongumine conjugates induce targeted protein degradation. Cell Chem Biol, 30(2):203-213.e17(2023) [pubmed] |
10.1016/j.chembiol.2023.01.004; |
Keyword List
submitter keyword: TurboID, PROTAC,PL, CDK9, E3 ligase |
Contact List
Dongwen Lv |
contact affiliation | Department of Biochemistry and Structural Biology and Center for Innovative Drug Discovery School of Medicine, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, United States |
contact email | lyud@uthscsa.edu |
lab head | |
Dongwen Lyu |
contact affiliation | UT Health San Antonio |
contact email | lyud@uthscsa.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD039401 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039401
- Label: PRIDE project
- Name: Identification of the E3 ligase(s) recruited by 955 to degrade CDK9