PXD039400 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming |
Description | Sexual reproduction of Toxoplasma gondii, which is restricted to the small intestine of felids, is sparsely documented in studies involving domestic cats, which also raises ethical concerns. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described. Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites, as revealed by mass spectrometry-based proteomic analyses of the parasites. |
HostingRepository | PRIDE |
AnnounceDate | 2023-09-16 |
AnnouncementXML | Submission_2023-09-16_07:19:49.286.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD039400 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | YohannCouté |
SpeciesList | scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-13 06:03:53 | ID requested | |
⏵ 1 | 2023-09-16 07:19:49 | announced | |
2 | 2024-01-29 06:53:15 | announced | 2024-01-29: Updated project metadata. |
Publication List
Keyword List
submitter keyword: merogony,Toxoplasma gondii, sexual commitment, Apetala, transcription factor, gene expression, nanoLC-MS/MS, epigenetic, chromatin |
Contact List
YohannCouté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
lab head | |
YohannCouté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD039400 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039400
- Label: PRIDE project
- Name: In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming