PXD039340-1

PXD039340 is an original dataset announced via ProteomeXchange.

Title	Confirmation of the subunit composition of Rhodobacter sphaeroides cytochrome bc1 complex isolated in styrene maleic acid nanodiscs.
Description	The purple α-proteobacterium Rhodobacter sphaeroides is a long-established model organism owing to its ability to switch between aerobic, anaerobic and phototrophic metabolism. This organism is also of great interest because of its phylogenetic relationship with the bacterial progenitor of the mitochondrion in eukaryotes. A component of the electron transport chain in both bacteria and mitochondria is the cytochrome bc1 (cytbc1) complex, a quinol:cytochrome c oxidoreductase that translocates protons across energy-transducing membranes. The resulting proton motive force is then utilized to drive ATP synthesis. Cytbc1 comprises three core subunits together with, in some organisms, one or more additional subunits. In Rhodobacter sphaeroides, the one additional subunit (subunit IV) is not fully characterized in terms of both structure and function. In this project, proteomic analysis is used to confirm the presence of all four subunits in a cytbc1 preparation isolated in SMA nanodiscs for structural analysis by cryo-EM.
HostingRepository	PRIDE
AnnounceDate	2023-03-14
AnnouncementXML	Submission_2023-03-14_04:38:13.535.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PhilipJackson
SpeciesList	scientific name: Rhodobacter sphaeroides 2.4.1; NCBI TaxID: 272943;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-09 07:38:23	ID requested
⏵ 1	2023-03-14 04:38:13	announced
2	2023-11-14 09:00:10	announced	2023-11-14: Updated project metadata.

submitter keyword: subunit IV,LC-MS/MS, Rhodobacter sphaeroides, purple bacteria, cytochrome bc1 complex, photosynthesis

DavidSwainsbury
contact affiliation	School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom.
contact email	d.swainsbury@uea.ac.uk
lab head
PhilipJackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039340
     1. Label: PRIDE project
     2. Name: Confirmation of the subunit composition of Rhodobacter sphaeroides cytochrome bc1 complex isolated in styrene maleic acid nanodiscs.