PXD039316-1

PXD039316 is an original dataset announced via ProteomeXchange.

Title	DeKinomics pulse-chases kinase functions in living cells
Description	Cellular context is crucial for understanding the complex and dynamic kinase functions in health and disease. Systematic dissection of kinase-mediated cellular processes requires rapid and precise stimulation (“pulse”) of a kinase of interest, as well as global and in-depth characterization (“chase”) of the perturbed proteome under living conditions. We herein developed an optogenetic “pulse-chase” strategy, termed Decaging Kinase coupled proteomics (DeKinomics), for proteome-wide profiling of kinase-driven phosphorylation at second-timescale in living cells. We took advantage of the “gain-of-function” feature of DeKinomics to identify direct kinase substrates and further portrayed the global phosphorylation of understudied receptor tyrosine kinases (RTKs) under native cellular settings. DeKinomics offered a general activation-based strategy to study kinase functions with high specificity and temporal resolution under living conditions.
HostingRepository	iProX
AnnounceDate	2023-10-07
AnnouncementXML	Submission_2023-12-10_20:15:45.680.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yicheng Weng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue; deaminated residue; iodoacetamide derivatized residue; methionine oxidation with neutral loss of 64 Da
Instrument	Q Exactive HF-X; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-08 20:04:34	ID requested
⏵ 1	2023-12-10 20:15:46	announced
2	2024-05-21 00:46:58	announced	2024-05-21: Update information.

Dataset with its publication pending

submitter keyword: Phosphoproteomics, pTyr proteomics

Ruijun Tian
contact affiliation	Southern University of Science and Technology
contact email	tianrj@sustech.edu.cn
lab head
Yicheng Weng
contact affiliation	Southern University of Science and Technology
contact email	wengyc12@qq.com
dataset submitter

iProX dataset URI