PXD039299-1
PXD039299 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome of CBR1 transgenic SK-OV-3 and OVCAR-2 cells |
| Description | Cell lysis samples containing 20 μg total protein determined by BCA method were precipitated with acetone. The precipitates were denatured with 50% trifluoroethanol. Disulfide bonds in proteins were reduced with DTT and alkylated with iodoacetamide followed by trypsin digestion. After desalting and purification of the resulting peptides, the samples were subjected to LC-MS/MS using a nanoLC system (Eksigent 400, AB Sciex) coupled online to a mass spectrometer (TripleTOF6600, AB Sciex). Database searching for acquired spectra was performed using a ProteinPilot 5.0.1 software (AB Sciex). Positive identification threshold was set at a false discovery rate of 1% or less. The resulting library file and SWATH (data independent acquisition) files were processed by PeakView (ver. 2.2.0, AB Sciex), and exported to MarkerView (ver. 1.3.0.1; AB Sciex). Peak areas of individual proteins were normalized relative to the sum of the peak areas of all detected proteins. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-04-04 |
| AnnouncementXML | Submission_2024-04-03_08:00:15.734.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yota Tatara |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; phosphorylated L-histidine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-01-06 01:00:47 | ID requested | |
| ⏵ 1 | 2024-04-03 08:00:16 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SK-OV-3, OVCAR-2, cancer, CBR1, SWATH |
Contact List
| Yoshihito Yokoyama | |
|---|---|
| lab head | |
| Yota Tatara | |
| contact affiliation | Hirosaki University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001979/ |
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