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PXD039299

PXD039299 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome of CBR1 transgenic SK-OV-3 and OVCAR-2 cells
DescriptionCell lysis samples containing 20 μg total protein determined by BCA method were precipitated with acetone. The precipitates were denatured with 50% trifluoroethanol. Disulfide bonds in proteins were reduced with DTT and alkylated with iodoacetamide followed by trypsin digestion. After desalting and purification of the resulting peptides, the samples were subjected to LC-MS/MS using a nanoLC system (Eksigent 400, AB Sciex) coupled online to a mass spectrometer (TripleTOF6600, AB Sciex). Database searching for acquired spectra was performed using a ProteinPilot 5.0.1 software (AB Sciex). Positive identification threshold was set at a false discovery rate of 1% or less. The resulting library file and SWATH (data independent acquisition) files were processed by PeakView (ver. 2.2.0, AB Sciex), and exported to MarkerView (ver. 1.3.0.1; AB Sciex). Peak areas of individual proteins were normalized relative to the sum of the peak areas of all detected proteins.
HostingRepositoryjPOST
AnnounceDate2024-04-04
AnnouncementXMLSubmission_2024-04-03_08:00:15.734.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYota Tatara
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; phosphorylated L-histidine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-06 01:00:47ID requested
12024-04-03 08:00:16announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SK-OV-3, OVCAR-2, cancer, CBR1, SWATH
Contact List
Yoshihito Yokoyama
lab head
Yota Tatara
contact affiliationHirosaki University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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