PXD039288-1

PXD039288 is an original dataset announced via ProteomeXchange.

Title	Oxygen-sensing through ERFVII action and modulation in Arabidopsis thaliana
Description	Oxygen is a key signalling component of plant biology and, whilst an oxygen-sensing mechanism was previously described in Arabidopsis thaliana. However, key features of the associated PCO N-degron pathway and Group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factor substrates remain unknown. We demonstrate that ERFVIIs show non-autonomous activation of root hypoxia tolerance, and are essential for root development and survival under oxygen limiting conditions in the soil. We determine the combined effects of ERFVIIs in controlling genome expression and define genetic and environmental components required for proteasome-dependent oxygen-regulated stability of ERFVIIs through the PCO N-degron pathway. Using a plant extract, unexpected amino-terminal cysteine oxidation to sulphonic acid oxidation level of ERFVIIs was defined, suggesting a requirement for additional enzymatic activity within the pathway. Our results provide a holistic understanding of the properties, functions and readouts of this oxygen-sensing mechanism defined through its role in modulating ERFVII stability.
HostingRepository	PRIDE
AnnounceDate	2023-07-02
AnnouncementXML	Submission_2023-07-02_03:56:12.208.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Neil Oldham
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-05 09:11:24	ID requested
⏵ 1	2023-07-02 03:56:12	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, N-degron pathway, cysteine oxidation

Professor NeilOldham
contact affiliation	School of Chemistry University of Nottingham
contact email	neil.oldham@nottingham.ac.uk
lab head
Neil Oldham
contact affiliation	University of Nottingham
contact email	neil.oldham@nottingham.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039288
     1. Label: PRIDE project
     2. Name: Oxygen-sensing through ERFVII action and modulation in Arabidopsis thaliana