PXD039287-1

PXD039287 is an original dataset announced via ProteomeXchange.

Title	Pcbp1 blocks MEK/ERK signaling and facilitate Naïve to Primed pluripotency
Description	Embryonic stem cells (ESCs) represent a unique cell type that is characterized by the ability to self-renew and differentiate into all types of somatic cells, as well as into germ cells. An understanding of the mechanisms which underlie their functioning is necessary for their usage in regenerative medicine. Pcbp1 is a DNA/RNA poly(C)-binding protein with a broad spectrum of functions including transcription, mRNA processing, and alternative splicing. In this study, we demonstrate that Pcbp1 blocks spontaneous ESCs differentiation towards primitive endoderm (PrE) by fine-tuning MEK/ERK signaling. Knockout of Pcbp1 leads to a specific PrE Oct4+Gata6+ population which is not receptive to differentiation signals. MEK/ERK inhibition prevents this phenotype but as our RNA-seq and Mass-spec data show, further Naïve-to-Primed transition is failed and leads to cell death, which is caused by the conflict of Primed and PrE gene expression network. Our results could explain an early lethal phenotype of Pcbp1-knockout mouse embryos and make clear the affected mechanisms. This dataset represents shotgun proteomics comparison of Mus musculus ESCs in Naïve to Primed states with or without Pcbp1 mutation.
HostingRepository	PRIDE
AnnounceDate	2026-06-04
AnnouncementXML	Submission_2026-06-04_10:17:59.004.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arseniy Lobov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-05 09:09:24	ID requested
⏵ 1	2026-06-04 10:17:59	announced

10.1016/J.STEMCR.2026.102950;

submitter keyword: pluripotency,Pcbp1, TimsToF Pro, embryonic stem cells, MEK/ERK signaling

Alexey Tomilin
contact affiliation	Head of the Laboratory of the Molecular Biology of Stem Cells, Institute of Cytology of the Russian Academy of Science, St. Petersburg, Russia
contact email	a.tomilin@incras.ru
lab head
Arseniy Lobov
contact affiliation	Yifat Merbl Lab, Dept. of Systems Immunology, Weizmann Institute of Science
contact email	arseniylobov@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD039287

PRIDE project URI

• PRIDE
  1. PXD039287
     1. Label: PRIDE project
     2. Name: Pcbp1 blocks MEK/ERK signaling and facilitate Naïve to Primed pluripotency