PXD039270-2

PXD039270 is an original dataset announced via ProteomeXchange.

Title	USING DESIGN OF EXPERIMENTS (DoE) TO OPTIMIZE PERFORMANCE AND STABILITY OF BIOMIMETIC CELL MEMBRANE-COATED NANOSTRUCTURES FOR CANCER THERAPY
Description	The present study aimed to develop and optimize cell derived membrane-coated nanostructures by applying a design of experiments (DoE) to improve the therapeutic index by homotypic targeting in cancer cells. The protein composition of the extracted cell membrane from tumoral cells were assessed by mass spectrometry-based proteomics. PLGA-based nanoparticles encapsulating temozolomide (TMZ NPs) were successfully developed. The coating technology applying the isolated U251 cell membrane (MB) was optimized using a fractional two-level three-factor factorial design. All the formulation runs were systematically characterized regarding their diameter, polydispersity index (PDI), and zeta potential (ZP). Experimental conditions generated by DoE were also subjected to morphological studies using negative-staining transmission electron microscopy (TEM). MicroRaman, Fourier-Transform Infrared (FTIR) spectroscopies and Confocal microscopy were used as characterization techniques for evaluating the NP-MB nanostructures. Internalization studies were carried out to evaluate the homotypic targeting ability. The results have shown that nearly 80% of plasma membrane proteins were retained in the cell membrane vesicles after the isolation process, including key proteins to the homotypic binding. DoE analysis considering acquired TEM images reveals that condition run five should be the best-optimized procedure to produce the biomimetic cell-derived membrane-coated nanostructure (NP-MB). Raman, FTIR, and confocal characterization results have shown the successful encapsulation of TMZ drug and provided evidence of the effective coating applying the MB. Cell internalization studies corroborate the proteomic data indicating that the optimized NP-MB achieved specific targeting of homotypic tumor cells. The structure should retain the complex biological functions of U251 natural cell membranes while exhibiting physicochemical properties suitable for effective homotypic recognition. Together, these findings provide coverage and a deeper understanding regarding the dynamics around extracted cell membrane and polymeric nanostructures interactions and an in-depth insight into the cell membrane coating technology and the development of optimized biomimetic and bioinspired nanostructured systems.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:07.052.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natália Ferreira
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-05 02:13:38	ID requested
1	2023-01-30 17:12:59	announced
⏵ 2	2023-11-14 09:00:07	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: cell membrane coating technology

mass spectrometry-based proteomics

biomimetic systems

stability

design of experiments

homotypic recognition

glioblastoma

temozolomide drug.

Valtencir Zucolotto
contact affiliation	Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, São Paulo University. Avenida Trabalhador São Carlense, 400, CEP13560970 (Brazil) Phone: (+55) 16 3373 8656, São Carlos – SP – Brazil.
contact email	zuco@ifsc.usp.br
lab head
Natália Ferreira
contact affiliation	Nanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, São Paulo University. Avenida Trabalhador São Carlense, 400, CEP13560970 (Brazil) Phone: (+55) 16 3373 8656, São Carlos – SP – Brazil.
contact email	natalia.noronha@usp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD039270

PRIDE project URI

• PRIDE
  1. PXD039270
     1. Label: PRIDE project
     2. Name: USING DESIGN OF EXPERIMENTS (DoE) TO OPTIMIZE PERFORMANCE AND STABILITY OF BIOMIMETIC CELL MEMBRANE-COATED NANOSTRUCTURES FOR CANCER THERAPY