PXD039229-1

PXD039229 is an original dataset announced via ProteomeXchange.

Title	Spatiotemporal and global profiling of DNA-protein interactions enables discovery of low-affinity transcription factors
Description	Precise dissection of DNA-protein interactions is essential for elucidating recognition basis, dynamics, and gene regulation mechanism. However, global profiling of weak and dynamic DNA-protein interactions remains a long-standing challenge. Here, we establish light-induced lysine (K) enabled crosslinking (LIKE-XL) strategy for spatiotemporal and global profiling of DNA-protein interactions. Harnessing the unique abilities to capture weak and transient DNA-protein interactions, we demonstrate that LIKE-XL enables discovery of low-affinity transcription factors (TFs)-DNA interactions via sequence-specific DNA baits, determining the binding sites for TFs previously unachieved. More importantly, we successfully decipher the dynamics of TF subproteome in response to drug treatment in time-resolved manner, and find new downstream target TFs from drug perturbations, providing insights into their dynamic transcriptional networks. The LIKE-XL offers a novel and complementary method to expand the DNA-protein profiling toolbox and map accurate DNA-protein interactomes previously inaccessible via noncovalent strategies, for better understanding of protein functions in health and disease.
HostingRepository	iProX
AnnounceDate	2023-01-03
AnnouncementXML	Submission_2023-01-03_19:59:49.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Linhui Zhai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-03 18:46:04	ID requested
⏵ 1	2023-01-03 19:59:49	announced
2	2023-05-07 21:15:11	announced	2023-05-08: Update publication information.

Dataset with its publication pending

submitter keyword: Spatiotemporal, DNA-protein interaction, crosslinking, transcription factor

Minjia Tan
contact affiliation	Shanghai Institute of Materia Medica
contact email	mjtan@simm.ac.cn
lab head
Linhui Zhai
contact affiliation	Shanghai Institute of Materia Medica
contact email	zhailinhui@simm.ac.cn
dataset submitter

iProX dataset URI