PXD039193-1

PXD039193 is an original dataset announced via ProteomeXchange.

Title	Effect of systemic, low-dose LPS treatment on brain proteome of PFF-inoculated mice
Description	Brain aggregates of α-synuclein (α-syn) characterizes a group of diseases defined as synucleinopathies. Recent studies implicate neuroinflammation as one of the central pathogenic mechanisms. However, how inflammation is linked to the α-syn pathology is still unknown. We wanted to address this by a ‘double-hit’ approach. Three weeks after intra-striatal injections of human α-syn pre-formed fibrils (PFF), mice were subjected for 3 weeks to repeated intraperitoneal injections of low concentrations (1 mg/ml) of lipopolysaccharide (LPS), a component of gram-negative bacteria. Histological analysis confirmed brain propagation of α-syn aggregation in PFF-injected mice independent of LPS-injections. No motor dysfunction was observed at this stage as measured in the pole test. Spleen immune cell profiling and multiplex cytokine analysis confirmed LPS-induced changes in T- and B-cells populations, monocytes, and neutrophils, and increased brain TNF-α, IL-β, IL-10 and KC/GRO levels. LC-MS/MS analysis in the forebrain area and subsequent downstream ReactomeGSA pathway analysis revealed that PFF-injections induced alterations in mitochondrial metabolism and neurotransmitter signaling. Systemic inflammation resulted in an overrepresentation of complement and coagulation pathways and upregulation of integrin and B cell receptor signaling in the PFF-injected mice.
HostingRepository	PRIDE
AnnounceDate	2024-09-05
AnnouncementXML	Submission_2024-09-05_04:51:14.006.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne-Line Laursen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 19:17:01	ID requested
⏵ 1	2024-09-05 04:51:14	announced
2	2024-10-22 06:57:45	announced	2024-10-22: Updated project metadata.

10.1016/J.MCN.2024.103931;

submitter keyword: alpha-synuclein, inflammation,LPS, PFF, LC-MS/MS

Susana Aznar
contact affiliation	Centre for Neuroscience & Stereology, Neurological Department, Bispebjerg Hospital, Denmark
contact email	susana.aznar.kleijn@regionh.dk
lab head
Anne-Line Laursen
contact affiliation	Roskilde University (DK), Bispebjerg Hospital (DK)
contact email	anne-line.strange.laursen@regionh.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD039193

PRIDE project URI

• PRIDE
  1. PXD039193
     1. Label: PRIDE project
     2. Name: Effect of systemic, low-dose LPS treatment on brain proteome of PFF-inoculated mice