PXD039155-1

PXD039155 is an original dataset announced via ProteomeXchange.

Title	A novel role for Teneurin C-terminal Associated Peptide (TCAP) in the regulation of cardiac activity in the Sydney rock oyster, Saccostrea glomerata
Description	Teneurin C-terminal associated peptide (TCAP) is an ancient bioactive peptide that is highly conserved in metazoans. TCAP administration reduces cellular and behavioural stress in vertebrate and urochordate models, yet despite numerous studies in higher animals, there is limited knowledge of its role in invertebrates. In particular, there are no studies on TCAP’s effects on the heart of any metazoan, which is a critical organ in the stress response. We used the Sydney rock oyster (SRO) as an invertebrate model to investigate a potential role for sroTCAP in regulating cardiac activity, including during stress. sroTCAP is localized to the neural innervation network of the SRO heart, and demonstrated binding with various heart proteins related to metabolism and stress, including SOD, GAPDH and metabotropic glutamate receptor. Intramuscular injection of sroTCAP (10 pmol) significantly altered the expression of heart genes that are known to regulate remodelling processes under different conditions, and modulated several gene families responsible for stress mitigation. sroTCAP (1 and 10 pmol) was shown to cause transient bradycardia (heart rate was reduced by up to 63% and for up to 40 min post-administration), indicative of an unstressed state. In summary, this study has established a role for a TCAP in the regulation of cardiac activity through modulation of physiological and molecular components associated with energy conservation, stress and adaptation. This represents a novel function for TCAP and may have implications for higher-order metazoans.
HostingRepository	PRIDE
AnnounceDate	2023-01-27
AnnouncementXML	Submission_2023-01-26_16:51:06.239.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TianfangWang
SpeciesList	scientific name: Saccostrea glomerata; NCBI TaxID: 157728;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	X500R QTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 01:58:52	ID requested
⏵ 1	2023-01-26 16:51:06	announced
2	2023-11-14 09:00:05	announced	2023-11-14: Updated project metadata.

submitter keyword: metabolism, transcriptome,teneurin, heart, TCAP, heart rate, oysters, stress

TianfangWang
contact affiliation	Centre for Bioinnovation, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia.
contact email	twang@usc.edu.au
lab head
TianfangWang
contact affiliation	University of the Sunshine Coast
contact email	twang@usc.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039155
     1. Label: PRIDE project
     2. Name: A novel role for Teneurin C-terminal Associated Peptide (TCAP) in the regulation of cardiac activity in the Sydney rock oyster, Saccostrea glomerata