PXD039151 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Blocking muscle wasting via deletion of the muscle specific E3 ligase MuRF1 impedes pancreatic tumor growth – ubiquitome dataset |
Description | Cancer-induced muscle wasting reduces quality of life, complicates or precludes cancer treatments, and predicts early mortality. Herein, we investigated the requirement of the muscle-specific E3 ubiquitin ligase, MuRF1, for muscle wasting induced by pancreatic cancer. Murine pancreatic cancer (KPC) cells, or saline, were injected into the pancreas of WT and MuRF1-/- mice, and tissues analyzed throughout tumor progression. KPC tumors induced progressive wasting of skeletal muscle and systemic metabolic reprogramming in WT mice, but not MuRF1-/- mice. KPC tumors from MuRF1-/- mice also grew slower, and showed an accumulation of metabolites normally depleted by rapidly growing tumors. Mechanistically, MuRF1 was necessary for the KPC-induced increases in cytoskeletal and muscle contractile protein ubiquitination, and the depression of proteins that support protein synthesis. Together, these data demonstrate that MuRF1 is required for KPC-induced skeletal muscle wasting, whose deletion reprograms the systemic and tumor metabolome and delays tumor growth. |
HostingRepository | PRIDE |
AnnounceDate | 2023-05-15 |
AnnouncementXML | Submission_2023-05-15_03:20:53.538.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | DariaNeyroud |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | ubiquitination signature dipeptidyl lysine |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-02 01:57:22 | ID requested | |
⏵ 1 | 2023-05-15 03:20:54 | announced | |
2 | 2023-11-14 08:48:21 | announced | 2023-11-14: Updated project metadata. |
Publication List
Neyroud D, Laitano O, Dasgupta A, Lopez C, Schmitt RE, Schneider JZ, Hammers DW, Sweeney HL, Walter GA, Doles J, Judge SM, Judge AR, Blocking muscle wasting via deletion of the muscle-specific E3 ligase MuRF1 impedes pancreatic tumor growth. Commun Biol, 6(1):519(2023) [pubmed] |
Keyword List
submitter keyword: proteasome system,Cancer cachexia, mus musculus, MuRF1, muscle wasting, pancreatic cancer |
Contact List
Andrew R.Judge |
contact affiliation | Department of Physical Therapy University of Florida |
contact email | arjudge@phhp.ufl.edu |
lab head | |
DariaNeyroud |
contact affiliation | University of Florida |
contact email | daria.neyroud@me.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039151
- Label: PRIDE project
- Name: Blocking muscle wasting via deletion of the muscle specific E3 ligase MuRF1 impedes pancreatic tumor growth – ubiquitome dataset