PXD039144-1

PXD039144 is an original dataset announced via ProteomeXchange.

Title	HeLa, HEK293T and mouse lung, heart and spleen LC-MS/MS
Description	Proteins are ubiquitously modified with glycans of varied chemical structures via distinct glycosidic linkages, making the landscape of protein glycosylation challenging to map. Profiling of intact glycopeptides with mass spectrometry (MS) has recently emerged as a powerful tool for revealing matched information of the glycosylation sites and attached glycans (i.e., intact glycosites), which, however, is largely limited to individual glycosylation types. Here, we develop Click-iG, which integrates metabolic labeling of glycans with clickable unnatural sugars, an optimized MS method, and a tailored version of pGlyco3 software to enable simultaneous enrichment and profiling of three types of—N-linked, O-linked, and O-GlcNAcylated—intact glycopeptides. We demonstrated Click-iG by identification of thousands of intact glycosites in cell lines and living mice. From the mouse lung, heart, and spleen, a total of 547 N-glycosylation sites with 161 glycan compositions, 198 O-glycosylation sites with 262 glycans, and 1,947 O‑GlcNAcylation sites were identified. The Click-iG-enabled unprecedent coverage on the protein glycosylation landscape lays the foundation for interrogating crosstalk between different glycosylation pathways.
HostingRepository	PRIDE
AnnounceDate	2025-02-12
AnnouncementXML	Submission_2025-02-12_08:33:25.600.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jialin Liu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 01:29:30	ID requested
⏵ 1	2025-02-12 08:33:26	announced

10.1002/anie.202303410;

Liu J, Cheng B, Fan X, Zhou X, Wang J, Zhou W, Li H, Zeng W, Yang P, Chen X, Click-iG: Simultaneous Enrichment and Profiling of Intact N-linked, O-GalNAc, and O-GlcNAcylated Glycopeptides. Angew Chem Int Ed Engl, 62(36):e202303410(2023) [pubmed]

submitter keyword: HeLa, mouse lung, heart, HEK293T, spleen, LC-MS/MS

xing chen
contact affiliation	PKU
contact email	xingchen@pku.edu.cn
lab head
Jialin Liu
contact affiliation	Peking University
contact email	jialinliu@pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD039144

PRIDE project URI

• PRIDE
  1. PXD039144
     1. Label: PRIDE project
     2. Name: HeLa, HEK293T and mouse lung, heart and spleen LC-MS/MS