PXD039133-1

PXD039133 is an original dataset announced via ProteomeXchange.

Title	Identification of gasderminD interacting proteins by mass spectrometry
Description	Gasdermin D (GSDMD)-mediated macrophage pyroptosis plays a critical role in inflammation and host defense. Plasma membrane perforation elicited by caspase-cleaved GSDMD N-terminal domain (GSDMD-NT) triggers membrane rupture and subsequent pyroptotic cell death, resulting in release of pro-inflammatory IL-1β and IL-18. Here, using a proteomics approach, we identified fatty acid synthase (FASN) as a GSDMD-binding partner and demonstrated that post-translational palmitoylation of GSDMD at Cys191/Cys192 (human/mouse) led to membrane translocation of GSDMD-NT but not full-length GSDMD. Collectively, we establish GSDMD-NT palmitoylation as a key regulatory mechanism modulating immune activity in infectious and inflammatory diseases.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:08:36.434.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039133
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arumugam Balasubramanian
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 01:20:58	ID requested
⏵ 1	2025-05-06 09:08:37	announced

10.1126/sciimmunol.adn1452;

10.6019/PXD039133;

Balasubramanian A, Hsu AY, Ghimire L, Tahir M, Devant P, Fontana P, Du G, Liu X, Fabin D, Kambara H, Xie X, Liu F, Hasegawa T, Xu R, Yu H, Chen M, Kolakowski S, Trauger S, Larsen MR, Wei W, Wu H, Kagan JC, Lieberman J, Luo HR, The palmitoylation of gasdermin D directs its membrane translocation and pore formation during pyroptosis. Sci Immunol, 9(94):eadn1452(2024) [pubmed]

submitter keyword: Fasn,Palmitoylation, GSDMD, Gasdermin D, Immunoprecipitation, Pyroptosis, LC-MSMS

Hongbo R Luo
contact affiliation	Department of Pathology, Dana-Farber/Harvard Cancer Center, Harvard Medical School; Department of Laboratory Medicine, Boston Children's Hospital, Enders Research Building, Room 811, Boston, MA, 02115, USA.
contact email	Hongbo.luo@childrens.havard.edu
lab head
Arumugam Balasubramanian
contact affiliation	Postdoc research fellow
contact email	arumugam.balasubramanian@childrens.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039133
     1. Label: PRIDE project
     2. Name: Identification of gasderminD interacting proteins by mass spectrometry