PXD039132 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Large-scale qualitative and quantitative research of dityrosine crosslinking omics in Escherichia coli |
Description | Excessive reactive oxygen species cause oxidative stress in cells. Two molecules of tyrosine can generate dityrosine, which is not only a product of natural posttranslational processes, but also a putative biomarker for protein oxidation. Few studies have investigated dityrosine crosslinking under endogenous or exogenous oxidative conditions in Escherichia coli at the proteome level so far. In this study, in order to investigate qualitative and quantitative dityrosine crosslinking, two mutant strains were constructed as endogenous oxidative stress model, as well as one mutant strain added with 1mM H2O2 as exogenous oxidative stress model. We integrated label-free LC-MS/MS and bioinformatics to research dityrosine crosslinking in Escherichia coli. A large-scale dataset of dityrosine crosslinking was provided. 352 proteins were identified to participate in dityrosine crosslinking by pLink. The subcellular localization included cytoplasmic (61.93%), periplasmic (21.31%), outer membrane (7.95%), inner membrane (5.97%) and extracellular (2.84%). Quantitative analysis of dityrosine crosslinking indicated that the three oxidative stress models could be separated at dityrosine linked peptides level. We expect that our research will guide efforts to comprehend the detailed features of dityrosine crosslinking in future. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:47:24.248.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhou Xiangzhe |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-02 01:20:19 | ID requested | |
1 | 2023-05-10 14:23:37 | announced | |
⏵ 2 | 2023-11-14 08:47:25 | announced | 2023-11-14: Updated project metadata. |
Publication List
Zhou X, Liu F, Li N, Zhang Y, . Antioxidants (Basel), 12(4):(2023) [pubmed] |
Keyword List
submitter keyword: Hydrogen peroxide,Dityrosine crosslinking, Label-free proteomics |
Contact List
Yongqian Zhang |
contact affiliation | School of Life Science, Beijing Institute of Technology |
contact email | zyq@bit.edu.cn |
lab head | |
Zhou Xiangzhe |
contact affiliation | Beijing Institute of Technology |
contact email | 3120211440@bit.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039132
- Label: PRIDE project
- Name: Large-scale qualitative and quantitative research of dityrosine crosslinking omics in Escherichia coli