PXD039127-1

PXD039127 is an original dataset announced via ProteomeXchange.

Title	Mapping the cytosolic proteins of HEK293T cells
Description	N-terminal proteoforms stem from the same gene but differ at their N-terminus, and most of these are found to be truncated, though some are N-terminally extended caused by ribosomes starting translation from codons in the annotated 5’UTR, and/or carry modified N-termini different from those of the canonical protein. Biological functions of N-terminal proteoforms are emerging, however, it remains unknown to what extend N-terminal proteoforms further expand the functional complexity. To address this in a more global manner, we mapped the interactomes of several pairs of N-terminal proteoforms and their canonical counterparts. For this, we first generated an in-depth catalogue of N-terminal proteoforms in the cytosol of HEK293T cells. In order to aid the selection of pairs of N-terminal proteoforms we generated a cytosolic map to verify their cytosolic interactions. This localization is crucial as Virotrap will be used for interactome analysis and this method works best for cytosolic proteins.
HostingRepository	PRIDE
AnnounceDate	2023-06-08
AnnouncementXML	Submission_2023-06-08_01:30:54.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AnneliesBogaert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-02 01:14:32	ID requested
⏵ 1	2023-06-08 01:30:55	announced
2	2023-11-14 09:01:07	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:47:17	announced	2024-10-22: Updated project metadata.

submitter keyword: cytosol,Fractionation, HEK293T cells, LC-MS/MS

KrisGevaert
contact affiliation	VIB Center for Medical Biotechnology, VIB, Ghent, B9052, Belgium Department of Biomolecular Medicine, Ghent University, Ghent, B9052, Belgium
contact email	kris.gevaert@vib-ugent.be
lab head
AnneliesBogaert
contact affiliation	VIB - Center for medical Biotechnology Ghent University - Department of Biomolecular Medicine
contact email	Annelies.bogaert@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD039127

PRIDE project URI

• PRIDE
  1. PXD039127
     1. Label: PRIDE project
     2. Name: Mapping the cytosolic proteins of HEK293T cells