PXD039063 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of CD24 interaction partners in urologic tumor cell lines |
Description | Being involved in adhesion, migration, and invasion, the highly glycosylated signal transducer CD24 (cluster of differentiation 24) has been implicated to play an essential role during carcinogenesis. Previously, the molecular and (epi)genetic regulation of CD24 has been characterized in testicular germ cell tumors (GCT). Here, CD24 was exclusively found in embryonal carcinoma (EC), which represents the stem cell like population of GCT (see project PXD025110). For a better understanding of the molecular function of CD24, this study aimed at the identification of the direct interaction partners of CD24 not only in GCTs, but also in other urologic malignancies, such as urothelial- (UC), prostate- (PC), and renal cell carcinoma (RCC). For this purpose, co-immunoprecipitations of CD24 were performed in GCT, UC, PC, and RCC cell lines, while CD24-deficient EC cells as well as IgG2a controls were included for high validity. Extracted proteome was measured by liquid-chromatography coupled with mass spectrometry (LS-MS). |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:02.788.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anja Stefanski |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-22 02:25:45 | ID requested | |
1 | 2023-05-10 17:33:37 | announced | |
⏵ 2 | 2023-11-14 08:48:07 | announced | 2023-11-14: Updated project metadata. |
Publication List
Skowron MA, Kotthoff M, Bremmer F, Ruhnke K, Parmaksiz F, Richter A, K, ü, ffer S, Reuter-Jessen K, Pauls S, Stefanski A, Str, ö, bel P, St, ü, hler K, Nettersheim D, an antibody-drug-conjugate and studying therapy resistance of yolk-sac tumors to identify and screen specific therapeutic options. Mol Med, 29(1):40(2023) [pubmed] |
Keyword List
submitter keyword: Germ cell tumor, CD24, urothelial carcinoma, renal cell carcinoma, co-immunoprecipitation, prostate cancer |
Contact List
Kai Stühler |
contact affiliation | Molecular Proteomics Laboratory, Heinrich Heine University Düsseldorf, Düsseldorf, GermanyMolecular Proteomics Laboratory, Heinrich Heine University Düsseldorf, Düsseldorf, Germany |
contact email | kai.stuehler@hhu.de |
lab head | |
Anja Stefanski |
contact affiliation | Molecular Proteomics Laboratory |
contact email | anja.stefanski@hhu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039063
- Label: PRIDE project
- Name: Identification of CD24 interaction partners in urologic tumor cell lines