PXD039050-1

PXD039050 is an original dataset announced via ProteomeXchange.

Title	Benefit of circadian clocks in adaptive immunity and vaccination responses
Description	The adaptive immune response is under circadian control, yet, the benefit of this rhythmicity for the organism is unknown. Furthermore, it is not understood why adaptive immune reactions continue to exhibit circadian changes over long periods of time. Using a combination of experimental and mathematical modelling approaches, we show here that dendritic cells (DCs) migrate from the skin to the draining lymph node (LN) in a time-of-day-dependent manner, which provides an enhanced likelihood for functional interactions with T cells. Greater numbers of infiltrating DCs induce rhythmic expression of TNF-α in the draining LN, which enhances ICAM-1 expression in high endothelial venules (HEVs), resulting in lymphocyte infiltration and LN expansion. Icam1 is controlled by binding of the core circadian clock transcription factor BMAL1 to its promoter region, and rhythmic Icam1 expression is lost in mice lacking endothelial cell BMAL1. LN cellularity continues to be different for weeks after the initial time-of-day-dependent challenge, which determines the immune response to vaccinations directed against Hepatitis A virus as well as SARS-CoV-2, as evidenced by rhythmic T cell reactions, germinal center formation and antibody production. Our results provide the mechanistic understanding of the time-of-day dependent development and maintenance of an adaptive immune response, demonstrating its dependency upon the timing of the initial challenge and the interactions of rhythmicity in multiple parameters. This provides a strategy for using time-of-day to optimize vaccination regimes.
HostingRepository	PRIDE
AnnounceDate	2023-02-08
AnnouncementXML	Submission_2023-02-08_06:50:34.286.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MarioOroshi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-22 01:27:28	ID requested
⏵ 1	2023-02-08 06:50:34	announced
2	2023-11-14 08:58:52	announced	2023-11-14: Updated project metadata.

submitter keyword: mathematical modeling, adaptive immunity, SARS-CoV-2, BMAL1, Hepatitis A,Circadian rhythm, vaccination, leukocyte trafficking

ChristophScheiermann
contact affiliation	BioMedizinisches Centrum Walter-Brendel-Zentrum für Experimentelle Medizin Ludwig-Maximilians-Universität München
contact email	christoph.scheiermann@med.uni-muenchen.de
lab head
MarioOroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039050

PRIDE project URI

• PRIDE
  1. PXD039050
     1. Label: PRIDE project
     2. Name: Benefit of circadian clocks in adaptive immunity and vaccination responses