PXD039021-2

PXD039021 is an original dataset announced via ProteomeXchange.

Title	Pupylation-independent substrate recruitment to proteasomal degradation in mycobacteria
Description	Mycobacteria and other Actinobacteria possess proteasomal degradation pathways in addition to the common bacterial compartmentalizing protease systems. Proteasomal degradation supports survival of these bacteria in adverse environments and conditions. The mycobacterial proteasome interacts with multiple ring-shaped activators, including the bacterial proteasome activator (Bpa), which facilitates the energy-independent degradation of heat shock repressor HspR in the successful human pathogen M. tuberculosis. To explore if this observation can be corroborated in the soil bacterium M. smegmatis, we performed an enrichment study in a bpa knockout in M. smegmatis. We grew the wild type M. smegmatis strain and the bpa knockout strain in biological triplicates under standard conditions or under heat shock and analyzed proteins which increased in abundance by data-independent acquisition mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:42.851.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Monika Pepelnjak
SpeciesList	scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-21 01:09:42	ID requested
1	2023-08-04 05:41:41	announced
⏵ 2	2024-10-22 05:59:43	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: heat shock,Mycobacteria, quality control, proteasomal degradation

Eilika Weber-Ban
contact affiliation	eilika@mol.biol.ethz.ch
contact email	eilika@mol.biol.ethz.ch
lab head
Monika Pepelnjak
contact affiliation	ETH Zurich
contact email	pepelnjak@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD039021

PRIDE project URI

• PRIDE
  1. PXD039021
     1. Label: PRIDE project
     2. Name: Pupylation-independent substrate recruitment to proteasomal degradation in mycobacteria