PXD038987-1

PXD038987 is an original dataset announced via ProteomeXchange.

Title	Differentiated protein extracts from freshwater and terrestrial mollusks inhibit virulence factor production in Cryptococcus neoformans
Description	Cryptococcus neoformans is a fungal pathogen responsible for an increased mortality among immunocompromised individuals. Long antifungal therapies to treat cryptococcal infections have compounded the occurrence of resistant strains that threaten the efficacy of current treatments. In this senseDue to resistance mechanisms, discovery of compounds that inhibit virulence factors, rather than kill the fungus, have emerged as potential new strategies to combat infection and reduce the rate of resistance due to lower selective pressure. Invertebrates rely solely on an effective innate immune system to prevent infections, provide providing a potential one health approach for discovery of novel antifungal and antibacterial compounds. Here, we demonstrate a differentiated extraction of proteins from three mollusks (freshwater and terrestrial) and evaluate extract their effects against the growth and virulence factor production (thermotolerance, melanin, capsule, and biofilm) in C. neoformans. We show that clarified extracts of Planorbella pilsbryi have a fungicidal effect on cryptococcal cells in a comparable way to Fluconazolefluconazole. Similarly, crude and clarifiedall extracts of Cipangopaludina chinensis not only affects cryptococcal thermotolerance but also impairs biofilm and capsule production. In addition, incubation of C. neoformans with clarified extracts of Cepaea nemoralis extracts reduced capsule production. Using inhibitory activity of extracts against peptidases related with virulence factors and Quantitative quantitative proteomics arose distinct proteome signatures for each extract and proposed proteins driving the observed anti-virulence properties. Overall, this work demonstratesproves the potential of compounds derived from natural sources to inhibit virulence factor production in a clinically important fungal pathogen.
HostingRepository	PRIDE
AnnounceDate	2023-03-06
AnnouncementXML	Submission_2023-03-06_10:11:45.753.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DavierGutierrez-Gongora
SpeciesList	scientific name: Cepaea nemoralis; NCBI TaxID: 28835; scientific name: Planorbella pilsbryi; NCBI TaxID: 2823525; scientific name: Cipangopaludina chinensis; NCBI TaxID: 527796;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-20 20:08:09	ID requested
⏵ 1	2023-03-06 10:11:46	announced
2	2023-11-14 09:00:21	announced	2023-11-14: Updated project metadata.

submitter keyword: Cryptococcus neoformans, extracts, invertebrates, antifungals, quantitative proteomics, mollusks, virulence factors, One health

JenniferGeddes-McAlister
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph, Canada.
contact email	jgeddesm@uoguelph.ca
lab head
DavierGutierrez-Gongora
contact affiliation	University of Guelph
contact email	davier@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038987
     1. Label: PRIDE project
     2. Name: Differentiated protein extracts from freshwater and terrestrial mollusks inhibit virulence factor production in Cryptococcus neoformans