PXD038964-1

PXD038964 is an original dataset announced via ProteomeXchange.

Title	Comparative phyloproteomics identifies conserved plasmodesmal proteins
Description	Plasmodesmata are cytosolic bridges, lined by the plasma membrane and traversed by endoplasmic reticulum, connecting cells and tissues, and critical for many aspects of plant biology. While plasmodesmata are notoriously difficult to extract, tissue fractionation and proteomic analyses can yield valuable knowledge of their composition. Here we have generated two novel proteomes to expand tissue and taxonomic representation of plasmodesmata: one from mature Arabidopsis leaves and one from the moss Physcomitrium patens and leveraged these and existing data to perform a comparative analysis to identify evolutionarily conserved protein families that are associated with plasmodesmata. Thus, we identified b-1,3-glucanases, C2 lipid-binding proteins and tetraspanins as core plasmodesmal components that likely serve as essential structural or functional components. Our approach has not only identified elements of a conserved plasmodesmal proteome, but also demonstrated the added power offered by comparative analysis for recalcitrant samples. Conserved plasmodesmal proteins establish a basis upon which ancient plasmodesmal function can be further investigated to determine the essential roles these structures play in multicellular organism physiology in the green lineages.
HostingRepository	PRIDE
AnnounceDate	2023-01-13
AnnouncementXML	Submission_2023-01-13_02:01:16.346.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MatthewJohnston
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Physcomitrella patens subsp. patens (Moss); NCBI TaxID: 3218;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-20 06:47:02	ID requested
⏵ 1	2023-01-13 02:01:16	announced
2	2023-11-14 08:59:35	announced	2023-11-14: Updated project metadata.

submitter keyword: Arabidopsis,plasmodesmata, phyloproteomics, Physcomitrium

ChristineFaulkner
contact affiliation	Crop Genetics John Innes Centre Norfolk United Kingdom
contact email	christine.faulkner@jic.ac.uk
lab head
MatthewJohnston
contact affiliation	Crop Genetics, John Innes Centre, Norwich, United Kingdom
contact email	mattjstn@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD038964

PRIDE project URI

• PRIDE
  1. PXD038964
     1. Label: PRIDE project
     2. Name: Comparative phyloproteomics identifies conserved plasmodesmal proteins