PXD038939-2

PXD038939 is an original dataset announced via ProteomeXchange.

Title	DECODING PROTEIN METHYLATION FUNCTION WITH THERMAL STABILITY ANALYSIS
Description	Protein methylation is emerging as an important modification beyond epigenetics. However, systems-wide analyses of protein methylation function lag behind compared to other modifications. Recently, thermal stability analyses have been developed which provide a proxy of a protein functional status. Here, we show that diverse molecular and functional events closely linked to protein methylation can be revealed by the analysis of thermal stability. Using mouse embryonic stem cells (mESC) as a model system, we show that Prmt5 regulates numerous mRNA binding proteins that are enriched in intrinsically disordered regions and involved in liquid-liquid phase separation mechanisms. Our data show for instance that methylation of Prmt5 substrates promotes the formation of stress granules. Moreover, we reveal a novel non-canonical function of Ezh2 in mitotic chromosomes and the organization of the perichromosomal layer and identify Mki67 as a putative novel Ezh2 substrate involved in this process. Our approach provides an opportunity to systematically explore protein methylation function and represents a rich resource for understanding the role of this modification in pluripotency.
HostingRepository	PRIDE
AnnounceDate	2024-10-04
AnnouncementXML	Submission_2024-10-04_01:36:40.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Sayago Ferreira
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	asymmetric dimethyl-L-arginine; monomethylated residue; symmetric dimethyl-L-arginine
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 05:14:18	ID requested
1	2023-04-27 14:18:35	announced
⏵ 2	2024-10-04 01:36:41	announced	2024-10-04: Updated project metadata.

Sayago C, S, á, nchez-Wandelmer J, Garc, í, a F, Hurtado B, Lafarga V, Prieto P, Zarzuela E, Xim, é, nez-Emb, ú, n P, Ortega S, Meg, í, as D, Fern, á, ndez-Capetillo O, Malumbres M, Munoz J, Decoding protein methylation function with thermal stability analysis. Nat Commun, 14(1):3016(2023) [pubmed]

10.1038/s41467-023-38863-1;

ProteomeXchange project tag: Stems Cells (B/D-HPP)

submitter keyword: thermal stability,Protein methylation, mouse embryonic stem cells, Prmt5, Ezh2, PISA

Javier Munoz
contact affiliation	Cell Signaling and Clinical Proteomics Group, Biocruces Bizkaia Health Research Institute, 48903 Barakaldo (Spain)
contact email	javier.munozperalta@osakidetza.eus
lab head
Cristina Sayago Ferreira
contact affiliation	CNIO
contact email	cristina.sa.fe.91@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD038939

PRIDE project URI

• PRIDE
  1. PXD038939
     1. Label: PRIDE project
     2. Name: DECODING PROTEIN METHYLATION FUNCTION WITH THERMAL STABILITY ANALYSIS