PXD038921-1

PXD038921 is an original dataset announced via ProteomeXchange.

Title	Subcellular location defines GPCR signal transduction
Description	G protein-coupled receptors in intracellular organelles can be activated in response to membrane permeant ligands, which contributes to the diversity and specificity of agonist action. The opioid receptors (ORs) provide a striking example, where small molecule opioid drugs activate ORs in the Golgi apparatus within seconds of drug addition. To date, our knowledge on the signaling of intracellular GPCRs remains incomplete and it is unknown if the downstream events triggered by ORs in plasma membrane and Golgi apparatus differ. To address this gap, we analyzed OR-mediated phosphorylation changes in cells treated with SNC80 (signaling control) or ICI-SNC80 (Golgi-restricted signaling) for 5 min or 25 min. We show that OR activation in the plasma membrane or Golgi apparatus have strikingly different downstream effects on protein phosphorylation. The study delineates OR signal transduction with unprecedented resolution and reveals that the subcellular location defines the signaling effect promoted by opioid drugs.
HostingRepository	PRIDE
AnnounceDate	2023-04-25
AnnouncementXML	Submission_2023-04-25_14:25:21.729.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038921
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	LucieOberhauser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 03:34:25	ID requested
⏵ 1	2023-04-25 14:25:22	announced
2	2023-11-14 09:00:06	announced	2023-11-14: Updated project metadata.

10.6019/PXD038921;

submitter keyword: Phosphoproteomics, SNC80, Golgi,HEK293, Opioid, DOR, GPCR, LC-ESI-MS/MS

MiriamStoeber
contact affiliation	Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland
contact email	miriam.stoeber@unige.ch
lab head
LucieOberhauser
contact affiliation	University of Geneva
contact email	lucie.oberhauser@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD038921

PRIDE project URI

• PRIDE
  1. PXD038921
     1. Label: PRIDE project
     2. Name: Subcellular location defines GPCR signal transduction