PXD038895-1

PXD038895 is an original dataset announced via ProteomeXchange.

Title	Trans-Golgi protein TVP23B regulates host-microbe interactions via Paneth cell homeostasis and Goblet cell glycosylation
Description	A key feature in intestinal immunity is the dynamic intestinal barrier, which separates the host from resident and pathogenic microbiota through a mucus gel impregnated with antimicrobial peptides. The mechanisms underlying the maintenance and function of this intestinal barrier are not completely understood. Using a mouse forward genetic screen for defects of intestinal homeostasis, we have found a mutation in Tvp23b, which conferred susceptibility to chemically induced and infectious colitis. Golgi apparatus membrane protein TVP23 homolog B (TVP23B) is a transmembrane protein conserved from yeast to humans. In the intestine, the protein is localized to the epithelium and its deficiency in the hematopoietic extrinsic compartment was essential to the colitis phenotype. We found that TVP23B controls the homeostasis of Paneth cells and function of goblet cells in vivo, leading to a decrease in antimicrobial peptides as well as a more penetrable mucus layer. As a result, Tvp23b-/- mice displayed decreased barrier function and a loss of host-microbe separation. TVP23B-deficient colonocytes have a loss of core-3 O-glycosylation of colonic proteins, which is the major O-glycosylation present on gel forming mucins. TVP23B binds with another Golgi protein, YIPF6, which is similarly critical for intestinal homeostasis. The Golgi proteomes of YIPF6 and TVP23B-deficient colonocytes have a common deficiency of several critical glycosylation enzymes, including those necessary for core-3 glycosylation of mucins. TVP23B is necessary for the formation of the sterile mucin layer of the intestine and its absence disturbs the balance of host and microbe in vivo.
HostingRepository	PRIDE
AnnounceDate	2023-07-20
AnnouncementXML	Submission_2023-07-20_11:13:28.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LiisaArike
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 02:47:44	ID requested
⏵ 1	2023-07-20 11:13:29	announced
2	2023-11-14 09:03:07	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:55:43	announced	2024-10-22: Updated project metadata.

Song R, McAlpine W, Fond AM, Nair-Gill E, Choi JH, Nystr, ö, m EEL, Arike L, Field S, Li X, SoRelle JA, Moresco JJ, Moresco EMY, Yates JR, Azadi P, Ni J, Birchenough GMH, Beutler B, Turer EE, Trans-Golgi protein TVP23B regulates host-microbe interactions via Paneth cell homeostasis and Goblet cell glycosylation. Nat Commun, 14(1):3652(2023) [pubmed]

submitter keyword: glycosylation, N-ethyl-N-nitrosourea (ENU), inflammatory bowel disease (IBD), goblet cel, Paneth Cell,Dextran sodium sulfate (DSS)

LiisaArike
contact affiliation	Mass Spectrometry Laboratory, Mucin Biology Groups,Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden
contact email	liisa.arike@gmail.com
lab head
LiisaArike
contact affiliation	PhD
contact email	liisaarike@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038895
     1. Label: PRIDE project
     2. Name: Trans-Golgi protein TVP23B regulates host-microbe interactions via Paneth cell homeostasis and Goblet cell glycosylation