PXD038884-1
PXD038884 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | 4D label free-based quantitative proteomic analysis of Eimeria tenella unsporulated oocysts and sporulated oocysts |
Description | We performed 4D label free-based quantitative proteomic analysis of Eimeria tenella unsporulated oocysts and sporulated oocysts. Briefly, protein extraction was performed using liquid nitrogen grinding and sonication on ice in SDT buffer (4% SDS, 100 mM Tris-HCl, pH7.6). After trypsin digestion, the peptides were separated on Easy-nLC1000 liquid chromatograph (Thermo Fisher Scientific, Waltham, MA, USA) with a trap column (Thermo Fisher Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) and analytical column (Thermo Fisher Scientific Easy Column, 25 cm long, 75 μm inner diameter, 1.9 μm resin). LC-MS/MS analysis was conducted on a timsTOF Pro mass spectrometry. The resulting LC-MS/MS data files were processed with the MaxQuant software (version 1.5.3.17) for identification and quantitation analysis. |
HostingRepository | iProX |
AnnounceDate | 2022-12-18 |
AnnouncementXML | Submission_2022-12-18_19:50:01.121.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Qing Liu |
SpeciesList | scientific name: Eimeria tenella; NCBI TaxID: 5802; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-12-18 19:49:40 | ID requested | |
⏵ 1 | 2022-12-18 19:50:01 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Eimeria tenella, proteomic analysis, unsporulated oocysts, sporulated oocysts |
Contact List
Xingquan Zhu | |
---|---|
contact affiliation | Shanxi Agricultural University |
contact email | xingquanzhu1@hotmail.com |
lab head | |
Qing Liu | |
contact affiliation | Shanxi Agricultural University |
contact email | lqsxau@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |