PXD038879-1

PXD038879 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Composition of minor ampullate silk makes its properties different from those of major ampullate silk
Description	Spider’s minor ampullate silk, or MI-silk, exhibits distinct mechanical properties and water resistance compared to its major ampullate counterpart (MA-silk). The principal protein constituent of MI-silk is known as minor ampullate spidroin, or MiSp, and while its sequence has been deciphered and is thought to underlie the differences in properties with MA-silk, the composition of MI-silk and the relationship between its composition and properties remain elusive. In this study, we set out to investigate the mechanical properties, water resistance, and proteome of MA-silk and MI-silk from Araneus ventricosus and Trichonephila clavata. We also synthesized artificial fibers from major ampullate spidroin, MaSp1 and 2, and MiSp to compare their properties. Our proteomic analysis reveals that the MI-silk of both araneids is composed of MiSp, MaSp1, and spidroin constituting elements (SpiCEs). The absence of MaSp2 in the MI-silk proteome and the comparison of the water resistance of artificial fibers suggest that the presence of MaSp2 is the reason for the disparity in water resistance between MI-silk and MA-silk.
HostingRepository	jPOST
AnnounceDate	2023-12-18
AnnouncementXML	Submission_2023-12-17_07:00:15.102.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038879
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Masaru Mori
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; Acetyl; L-methionine sulfoxide
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-18 05:46:22	ID requested
⏵ 1	2023-12-17 07:00:15	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Araneus ventricosus, Trichonephila clavata, dragline silk, spider silk

submitter keyword: Araneus ventricosus, Trichonephila clavata, dragline silk, spider silk

Contact List

Kazuharu Arakawa
lab head
Masaru Mori
contact affiliation	Institute for Advanced Biosciences, Keio University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001957/