PXD038856-1

PXD038856 is an original dataset announced via ProteomeXchange.

Title	Functional nanovesicles from human trophectodermal cells for embryo implantation
Description	Extracellular vesicles (EVs) are important mediators of embryo attachment and outgrowth critical for successful implantation. While EVs have garnered immense interest in their therapeutic potential in assisted reproductive technology by improving implantation success, their large-scale generation remains a major challenge. Here, we report a rapid and scalable production of nanovesicles (NVs) from human trophectoderm cells (hTSCs) via membrane extrusion; these NVs can be generated in approximately 6 hours with a 20-fold higher yield than EVs isolated from the same number of cells. NVs display similar biophysical traits (morphologically intact, spherical, 90-130 nm) to EVs, and are laden with hallmark players of implantation (ITGA2/V, ITGB1, PRDX1, SOD2, and MFGE8) that include cell-matrix adhesion and extracellular matrix organisation proteins. Functionally, NVs are readily taken up by low-receptive endometrial HEC1A cells and reprogram their proteome towards a receptive phenotype that support hTSC spheroid attachment. Moreover, a single dose treatment of NVs significantly enhanced adhesion and spreading of mouse embryo trophoblast on fibronectin matrix. We show functional potential of NVs in enhancing embryo implantation and highlight their rapid and scalable generation, amenable to clinical utility.
HostingRepository	PRIDE
AnnounceDate	2023-02-20
AnnouncementXML	Submission_2023-02-19_16:40:35.491.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	davidgreening
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-14 22:13:06	ID requested
⏵ 1	2023-02-19 16:40:35	announced

Dataset with its publication pending

submitter keyword: proteomics, extrusion,Nanovesicles, extracellular vesicles, embryo implantation, endometrial receptivity

DavidGreening
contact affiliation	Baker Heart & Diabetes Inst.
contact email	david.greening@baker.edu.au
lab head
davidgreening
contact affiliation	Baker Heart and Diabetes Inst.
contact email	david.greening@baker.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038856
     1. Label: PRIDE project
     2. Name: Functional nanovesicles from human trophectodermal cells for embryo implantation