PXD038848-1

PXD038848 is an original dataset announced via ProteomeXchange.

Title	An apicomplexan bromodomain, TgBDP1 associates with diverse epigenetic factors to regulate essential transcriptional processes in Toxoplasma gondii.
Description	The protozoan pathogen Toxoplasma gondii relies on tight regulation of gene expression to invade and establish infection in its host. The divergent gene regulatory mechanisms of Toxoplasma and related apicomplexan pathogens rely heavily on regulators of chromatin structure and histone modifications. The important contribution of histone acetylation for Toxoplasma in both acute and chronic infection has been demonstrated, where histone acetylation increases at active gene loci. However, the direct consequences of specific histone acetylation marks and the signaling pathway that influences transcriptional regulation in response to the modification is unclear. As a reader of lysine acetylation, the bromodomain serves as a mediator between the acetylated histone and transcriptional regulators. Here we show that the bromodomain protein TgBDP1 which is conserved amongst Apicomplexa and within the Alveolata superphylum, is essential for Toxoplasma asexual proliferation. Using CUT&TAG we demonstrate that TgBDP1 is recruited to transcriptional start sites of a large proportion of parasite genes. Transcriptional profiling during TgBDP1 knockdown revealed that loss of TgBDP1 leads to major dysregulation of gene expression, implying multiple roles for TgBDP1 in both gene activation and repression. This is supported by interactome analysis of TgBDP1 demonstrating that TgBDP1 forms a core complex with two other bromodomain proteins and an ApiAP2 factor. This core complex appears to interact with other epigenetic factors such as nucleosome remodelling complexes. We conclude that TgBDP1 interacts with diverse epigenetic regulators to exert opposing influences on gene expression in the Toxoplasma tachyzoite.
HostingRepository	PRIDE
AnnounceDate	2023-06-01
AnnouncementXML	Submission_2023-05-31_18:02:50.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SethMcNutt
SpeciesList	scientific name: Toxoplasma gondii ME49; NCBI TaxID: 508771;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-14 22:05:52	ID requested
⏵ 1	2023-05-31 18:02:51	announced

Dataset with its publication pending

submitter keyword: Toxoplasma, Gondii, Transcriptional, Bromodomain, Apicomplexan, Histone, Epigenetic, TgBDP1, Acetylation, LC-MS/MS

FeixiaChu
contact affiliation	Department of Molecular, Cellular, & Biomedical Sciences, Chu Lab, University of New Hampshire, United States
contact email	feixia.chu@unh.edu
lab head
SethMcNutt
contact affiliation	University of New Hampshire
contact email	seth.mcnutt@unh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038848
     1. Label: PRIDE project
     2. Name: An apicomplexan bromodomain, TgBDP1 associates with diverse epigenetic factors to regulate essential transcriptional processes in Toxoplasma gondii.