PXD038825-1

PXD038825 is an original dataset announced via ProteomeXchange.

Title	Being spontaneous has its costs! Characterization of the spontaneous phage ϕD5-resistant mutants of Dickeya solani strain IPO 2222
Description	Lytic bacteriophages able to infect and kill Dickeya spp. can be readily isolated from virtually all Dickeya spp.-containing environments, yet little is known about the selective pressure those viruses exert on their hosts. Here, we identified two spontaneous phage-resistant D. solani IPO 2222 mutants, DsR34 and DsR207, resistant to infection caused by phage vB_Dsol_D5 (ΦD5) that expressed a reduced ability to macerate potato tuber tissues compared to the wild-type, phage-susceptible D. solani IPO 2222 strain. Genome sequencing revealed that mutants had point mutations in two genes encoding: secretion protein HlyD (mutant DsR34) and elongation factor Tu (EF-Tu) (mutant DsR207). Both mutations impacted the proteoms of D. solani grown in rich and minimal media. Furthermore, DsR34 and DsR207 were characterized for features essential for their ecological success in a plant environment, including the ability to use various carbon and nitrogen sources, production of plant cell wall degrading enzymes, ability to form biofilms, siderophore production, swimming and swarming motility and virulence in planta. Compared to the wild-type ΦD5-susceptible D. solani strain, mutants DsR34 and DsR207 expressed reduced ability to macerate chicory leaves and to colonize and cause symptoms in growing potato plants. The implications of the ΦD5 resistance on the ecological performance of D. solani are discussed.
HostingRepository	PRIDE
AnnounceDate	2023-04-28
AnnouncementXML	Submission_2023-04-28_04:37:12.703.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	InezMruk
SpeciesList	scientific name: Dickeya solani IPO 2222; NCBI TaxID: 1225786;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-13 23:24:06	ID requested
⏵ 1	2023-04-28 04:37:13	announced

Dataset with its publication pending

submitter keyword: Erwinia chrysanthemi, environmental fitness,phage resistance, fitness costs, ecology, potato

Paulina Czaplewska, Assoc.Prof.
contact affiliation	Lab head of Mass Spectrometry Laboratory, Intercollegiate Faculty of Biotechnology University of Gdańsk and Medical University of Gdańsk, Abrahama 58, 80-307 Gdańsk, Poland
contact email	paulina.czaplewska@ug.edu.pl
lab head
InezMruk
contact affiliation	Intercollegiate Faculty of Biotechnology University of Gdańsk and Medical University of Gdańsk
contact email	inez.mruk@phdstud.ug.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038825
     1. Label: PRIDE project
     2. Name: Being spontaneous has its costs! Characterization of the spontaneous phage ϕD5-resistant mutants of Dickeya solani strain IPO 2222