PXD038824-1

PXD038824 is an original dataset announced via ProteomeXchange.

Title	A simple non-invasive method to collect soft tick saliva reveals differences in Ornithodoros moubata saliva composition between ticks infected and uninfected with Borrelia duttonii spirochetes
Description	We developed a new simple method to assess the composition of proteins in the Ornithodoros moubata saliva. To collect naturally expectorated saliva from the ticks, we employed an artificial membrane feeding technique using a simple, chemically defined diet and submitted the collected native saliva samples for liquid chromatography-mass spectrometry (LC-MS) analysis. These experiments were conducted with groups of uninfected ticks as well as with B. duttonii infected ticks. The ticks exhibited a fair feeding response to the tested diet, engorgement rates reaching between 60-100% of ticks per feeding chamber. LC-MS analysis identified a total of 17 and 15 proteins in saliva samples from the uninfected and infected ticks respectively. Importantly, the analysis was sensitive enough to detect up to 9 different proteins in the saliva containing diet upon which as few as 6 nymphal ticks fed. Some of the proteins identified are well known for their immunomodulatory activity in a vertebrate host, whereas others were structural or “housekeeping” proteins and their finding in the naturally expectorated saliva confirms that they can be secreted and having some roles at the tick-host interface. Most notably, some of the proteins that have long been suspected as important for vector-pathogen interactions of Borrelia spirochetes were detected only in the samples from infected ticks, suggesting that their expression was altered by the persistent colonization of the tick’s salivary glands by spirochetes. The simple method described herein is an important addition to the toolbox available to study the vector-host-pathogen interactions in the rapidly feeding soft ticks.
HostingRepository	PRIDE
AnnounceDate	2023-01-10
AnnouncementXML	Submission_2023-01-10_08:33:25.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FilipDyčka
SpeciesList	scientific name: Borrelia; NCBI TaxID: NCBITaxon:138; scientific name: Ornithodoros moubata; NCBI TaxID: 6938;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-13 23:23:46	ID requested
⏵ 1	2023-01-10 08:33:26	announced
2	2023-11-14 08:59:58	announced	2023-11-14: Updated project metadata.

submitter keyword: Relapsing fever Borrelia, artificial membrane feeding, Ornithodoros moubata, Saliva, infected,tick, LC-MS Analysis, Borrelia duttonii, spirochetes

Ryan O.Rego
contact affiliation	Institute of Parasitology, Centre for Biology, Academy of Science of the Czech Republic, Czech Republic Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic
contact email	ryanrego@paru.cas.cz
lab head
FilipDyčka
contact affiliation	University of South Bohemia in Ceske Budejovice Faculty of Science Branisovska 1760 Ceske Budejovice
contact email	dycka@mail.muni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038824
     1. Label: PRIDE project
     2. Name: A simple non-invasive method to collect soft tick saliva reveals differences in Ornithodoros moubata saliva composition between ticks infected and uninfected with Borrelia duttonii spirochetes