PXD038821-2

PXD038821 is an original dataset announced via ProteomeXchange.

Title	Endogenous TOM20 proximity labelling: a Swiss-knife for the study of mitochondrial proteins in human cells
Description	The majority of mitochondrial proteins are encoded by the nuclear genome and must be imported into the mitochondria. There are two main paths for mitochondrial protein import: post-translational and co-translational import. Co-translational import couples the translation and the translocation of the mitochondrial proteins, alleviating the energy cost typically associated with the post-translational import relying on chaperone systems. The mitochondrial co-translational import mechanisms are still unclear with few actors identified but none have been described in mammals yet. We thus profiled the TOM20 proxisome using BioID, assuming that some of identified proteins could be molecular actors of the co-translational import in human cells. The obtained results showed a high enrichment of RNA binding proteins close to the TOM complex. However, for the few selected candidates, we could not demonstrate a role in the mitochondrial co-translational import process. Nonetheless, we were able to demonstrate a new mitochondrial localization for nuclear proteins. Besides, additional analyses revealed a negative correlation between the abundance of mitochondrial proteins and their reported half-life. This experimental approach is thus proposed to potentially characterize mitochondrial co-translational import effectors in human cells and to monitor protein entry inside mitochondria with a potential application in the prediction of mitochondrial protein half-life.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:19.004.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038821
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Dieu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-13 23:22:35	ID requested
1	2023-07-20 11:24:15	announced
⏵ 2	2023-11-14 09:03:20	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:56:14	announced	2024-10-22: Updated project metadata.

Meurant S, Mauclet L, Dieu M, Arnould T, Eyckerman S, Renard P, Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells. Int J Mol Sci, 24(11):(2023) [pubmed]

10.6019/PXD038821;

submitter keyword: Mitochondria

Co-translational import

BioID

Protein identification

Mass spectrometry

Patricia Renard
contact affiliation	1 URBC, Namur Research Institute for Life Sciences (Narilis), University of Namur (UNamur), 5000, Namur, Belgium
contact email	patricia.renard@unamur.be
lab head
Marc Dieu
contact affiliation	University of Namur - MaSUN
contact email	marc.dieu@unamur.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD038821

PRIDE project URI

• PRIDE
  1. PXD038821
     1. Label: PRIDE project
     2. Name: Endogenous TOM20 proximity labelling: a Swiss-knife for the study of mitochondrial proteins in human cells