PXD038818 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | D2O proteome dynamics in FSHD vs UASb myoblasts |
Description | Proteomic studies in facioscapulohumeral muscular dystrophy (FSHD) could offer new insight to disease mechanisms underpinned by post-transcriptional processes. We used stable isotope (deuterium oxide; D2O) labelling and peptide mass spectrometry to investigate the abundance and turnover rates of proteins in cultured muscle cells from 2 individuals affected by FSHD and their unaffected siblings (UASb). We measured the abundance of 4483 proteins and the turnover rate of 2324 proteins in each (n = 4) myoblast sample. FSHD myoblasts exhibited a greater abundance but slower turnover rate of subunits of mitochondrial respiratory complexes and mitochondrial ribosomal proteins, which may indicate an accumulation of ‘older’ less viable mitochondrial proteins in myoblasts from individuals affected by FSHD. Our results highlight the importance of post-transcriptional processes and protein turnover in FSHD pathology and provide a resource for the FSHD research community to explore this burgeoning aspect of FSHD. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:01:40.627.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD038818 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jatin Burniston |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-13 23:21:30 | ID requested | |
1 | 2023-06-27 04:11:47 | announced | |
⏵ 2 | 2023-11-14 09:01:48 | announced | 2023-11-14: Updated project metadata. |
Publication List
Nishimura Y, Bittel AJ, Stead CA, Chen YW, Burniston JG, Facioscapulohumeral Muscular Dystrophy is Associated With Altered Myoblast Proteome Dynamics. Mol Cell Proteomics, 22(8):100605(2023) [pubmed] |
Keyword List
submitter keyword: Human, FUSION |
Contact List
Jatin G Burniston |
contact affiliation | Liverpool John Moores University |
contact email | j.burniston@ljmu.ac.uk |
lab head | |
Jatin Burniston |
contact affiliation | Liverpool John Moores University |
contact email | j.burniston@ljmu.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038818
- Label: PRIDE project
- Name: D2O proteome dynamics in FSHD vs UASb myoblasts