PXD038743-2

PXD038743 is an original dataset announced via ProteomeXchange.

Title	Analysis of the protein distribution within the wheat endosperm
Description	Starchy endosperm proteins determine wheat quality and exhibit, besides a quantitative protein gradient, a qualitative protein gradient from the outer to inner starchy endosperm. The goal was to investigate the relative differences in protein composition between the aleurone, sub-aleurone and inner endosperm. Using laser microdissection followed by nanoLC-MS/MS, an innovative method combining a high spatial specificity and analytical selectivity in sample-limited situations, 780 proteins were detected and classified by function. Relatively more gluten proteins were detected in the sub-aleurone compared to inner endosperm. Gluten composition-wise, the sub-aleurone is relatively more enriched in ω-gliadins, but impoverished in LMW-GS and γ-gliadins. While a basic set of albumins and globulins is detected across the entire endosperm, some proteins, like puroindoline-B, display an increasing (or decreasing) gradient. Histological origin and relative positioning of the endosperm cells are hypothesized to drive the protein gradient. Knowledge on this gradient provides major opportunities for the wheat manufacturing industry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:17:46.254.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sabrina Geisslitz
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-12 04:57:01	ID requested
1	2023-12-14 23:25:10	announced
⏵ 2	2024-10-22 06:17:46	announced	2024-10-22: Updated project metadata.

10.1016/J.FOODCHEM.2023.137735;

submitter keyword: Triticum aesticum,nanoLC-MS/MS, sub-aleurone, inner endosperm, laser microdessected, aleurone

Katharina Anne Scherf
contact affiliation	Department of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany
contact email	katharina.scherf@kit.edu
lab head
Sabrina Geisslitz
contact affiliation	Department of Bioactive and Functional Food Chemistry, Institut of Applied Biosciences, Karlsruhe Institut of Technology
contact email	sabrina.geisslitz@kit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD038743

PRIDE project URI

• PRIDE
  1. PXD038743
     1. Label: PRIDE project
     2. Name: Analysis of the protein distribution within the wheat endosperm