PXD038727-2

PXD038727 is an original dataset announced via ProteomeXchange.

Title	Upregulation of filaggrin 2 in scleritis using tissue mass spectrometry
Description	Scleritis is a severe inflammatory ocular disorder with unknown pathogenesis. Using a mass spectrometry approach, we investigated healthy sclera and sclera affected by non-infectious scleritis for differentially expressed proteins. Therefore, we collected scleral samples of enucleated eyes due to severe non-infectious scleritis (n=3) and control scleral tissues (n=5), all exenterated eyes for eyelid carcinomas without scleral invasion. Nano LC-MS mass spectrometry identified 629 proteins within the healthy and diseased scleral tissues, of which collagen type I was the most abundantly expressed protein. Collagen type II-XII was also present. Filaggrin-2, a protein that plays a crucial role in epidermal barrier function, was upregulated in all scleritis cases. In addition, other epithelial-associated proteins were upregulated (such as Keratin 33b, 34, and 85, Epiplakin, transglutaminase-3, Galectin 7, and Caspase-14) in scleritis. Further, upregulated proteins involved in regulation of the cytoskeleton (Vinculin and Myosin 9) and housekeeping proteins were found (elongation factor-2 and cytoplasmic dynein 1). We selected two proteins for validation with immunohistochemistry: Fillagrin-2 and myosin 9. Upregulation of both proteins was confirmed, the latter protein showing co-localization with the endothelial cell marker ETC-related gene (ERG), indicating neovascularization in scleral tissue affected in scleritis. Further research, preferably with multiple scleritis cases, is needed to validate our findings, including identification of the specific role of Filaggrin-2 and angiogenesis in the pathogenesis of scleritis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:13.134.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038727
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Coskun Guzel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-10 14:51:51	ID requested
1	2023-05-10 14:00:18	announced
⏵ 2	2023-11-14 08:47:21	announced	2023-11-14: Updated project metadata.

Vergouwen DPC, Ten Berge JC, Guzel C, van den Bosch TPP, Verdijk RM, Rothova A, Luider TM, Schreurs MWJ, Scleral Proteome in Noninfectious Scleritis Unravels Upregulation of Filaggrin-2 and Signs of Neovascularization. Invest Ophthalmol Vis Sci, 64(3):27(2023) [pubmed]

10.6019/PXD038727;

submitter keyword: tissue,Scleritis, nano-LCMS, DDA

Dr. T.M. Luider
contact affiliation	Head of Laboratory Neuro-Oncology Clinical and Cancer Proteomics Neurology
contact email	t.luider@erasmusmc.nl
lab head
Coskun Guzel
contact affiliation	Erasmus MC
contact email	c.guzel@erasmusmc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038727
     1. Label: PRIDE project
     2. Name: Upregulation of filaggrin 2 in scleritis using tissue mass spectrometry