PXD038660-2

PXD038660 is an original dataset announced via ProteomeXchange.

Title	Aging-triggered iron-rich insoluble protein aggregates in the spleen originate chiefly from red pulp macrophages.
Description	Both iron homeostasis and erythropoiesis are known to be affected by aging. Iron needs in mammals are met primarily by iron recycling from senescent red blood cells (RBCs), a task chiefly accomplished by red pulp macrophages (RPMs) in the spleen. Given that RPMs continuously process iron, their cellular functions might be susceptible to age-dependent decline, a possibility that has been unexplored to date. In our project, we identified a formation of undegradable iron- and heme-rich extracellular aggregates in the spleens of 10-11-month-old female mice. To better understand the origin of these aggregates, here, we performed: i) protein identification and intrasample quantification (iBAQ) of proteins of magnetically-isolated red pulp macrophages from spleens of two female 8-weeks-old C57BL/6J (maintained on a standard diet) and ii) label-free quantification of proteins of the splenic protein aggregates formed in the mouse spleen 24 hours after intraperitoneal iron dextran injection, using dextran-injected mice as a control. Two 8-week-old C57BL/6J mice per group were analyzed. This dataset is related to the project PXD032900, which describes quanytitative analysis of proteins in aggregates magnetically isolated from spleen of aged (standard or iron-reduced diet) and young mice (standard diet).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:22:35.176.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 12:17:01	ID requested
1	2023-02-02 00:43:06	announced
⏵ 2	2023-11-14 07:22:37	announced	2023-11-14: Updated project metadata.

Slusarczyk P, Mandal PK, Zurawska G, Niklewicz M, Chouhan K, Mahadeva R, Jo, ń, czy A, Macias M, Szybinska A, Cybulska-Lubak M, Krawczyk O, Herman S, Mikula M, Serwa R, Lenartowicz M, Pokrzywa W, Mleczko-Sanecka K, Impaired iron recycling from erythrocytes is an early hallmark of aging. Elife, 12():(2023) [pubmed]

submitter keyword: iron-rich protein aggregates, iron-dextran injection,Mouse, red pulp macrophages, spleen

Dr. Katarzyna Mleczko-Sanecka
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	kmsanecka@iimcb.gov.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038660
     1. Label: PRIDE project
     2. Name: Aging-triggered iron-rich insoluble protein aggregates in the spleen originate chiefly from red pulp macrophages.