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PXD038660

PXD038660 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAging-triggered iron-rich insoluble protein aggregates in the spleen originate chiefly from red pulp macrophages.
DescriptionBoth iron homeostasis and erythropoiesis are known to be affected by aging. Iron needs in mammals are met primarily by iron recycling from senescent red blood cells (RBCs), a task chiefly accomplished by red pulp macrophages (RPMs) in the spleen. Given that RPMs continuously process iron, their cellular functions might be susceptible to age-dependent decline, a possibility that has been unexplored to date. In our project, we identified a formation of undegradable iron- and heme-rich extracellular aggregates in the spleens of 10-11-month-old female mice. To better understand the origin of these aggregates, here, we performed: i) protein identification and intrasample quantification (iBAQ) of proteins of magnetically-isolated red pulp macrophages from spleens of two female 8-weeks-old C57BL/6J (maintained on a standard diet) and ii) label-free quantification of proteins of the splenic protein aggregates formed in the mouse spleen 24 hours after intraperitoneal iron dextran injection, using dextran-injected mice as a control. Two 8-week-old C57BL/6J mice per group were analyzed. This dataset is related to the project PXD032900, which describes quanytitative analysis of proteins in aggregates magnetically isolated from spleen of aged (standard or iron-reduced diet) and young mice (standard diet).
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:22:35.176.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRemigiusz Serwa
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-07 12:17:01ID requested
12023-02-02 00:43:06announced
22023-11-14 07:22:37announced2023-11-14: Updated project metadata.
Publication List
Slusarczyk P, Mandal PK, Zurawska G, Niklewicz M, Chouhan K, Mahadeva R, Jo, ń, czy A, Macias M, Szybinska A, Cybulska-Lubak M, Krawczyk O, Herman S, Mikula M, Serwa R, Lenartowicz M, Pokrzywa W, Mleczko-Sanecka K, Impaired iron recycling from erythrocytes is an early hallmark of aging. Elife, 12():(2023) [pubmed]
Keyword List
submitter keyword: iron-rich protein aggregates, iron-dextran injection,Mouse, red pulp macrophages, spleen
Contact List
Dr. Katarzyna Mleczko-Sanecka
contact affiliationInternational Institute of Molecular and Cell Biology in Warsaw, Poland
contact emailkmsanecka@iimcb.gov.pl
lab head
Remigiusz Serwa
contact affiliationThe International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact emailr.serwa@imol.edu.pl
dataset submitter
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