PXD038631-1

PXD038631 is an original dataset announced via ProteomeXchange.

Title	Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET
Description	Gas Vesicles (GVs) are gas-filled protein nanostructures employed by several species of bacteria and archaea as flotation devices to enable access to optimal light and nutrients. The unique physical properties of GVs have led to their use as genetically-encodable contrast agents for ultrasound and MRI. Currently, however, the structure and assembly mechanism of GVs remain unknown. Here we employ cryo-electron tomography to reveal how the GV shell is formed by a helical filament of highly-conserved GvpA subunits. This filament changes polarity at the center of the GV cylinder, a site that may act as an elongation center. High-resolution subtomogram averaging reveals a corrugated pattern of the shell arising from polymerization of GvpA into a β-sheet. The accessory protein GvpC forms a helical cage around the GvpA shell, providing reinforcement. Together, our results help explain the remarkable mechanical properties of GVs and their ability to adopt different diameters and shapes.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_11:11:39.395.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting-Yu Wang
SpeciesList	scientific name: Dolichospermum flos-aquae 04-10; NCBI TaxID: 749200;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 06:47:26	ID requested
⏵ 1	2024-10-07 11:11:39	announced

Dataset with its publication pending

submitter keyword: cryo-ET.,Gas Vesicles

Grant J. Jensen
contact affiliation	Division of Biology and Biological Engineering, California Institute of Technology,USA
contact email	grant_jensen@byu.edu
lab head
Ting-Yu Wang
contact affiliation	Caltech
contact email	tywang@caltech.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038631
     1. Label: PRIDE project
     2. Name: Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET