PXD038623-1

PXD038623 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The role of DENND10 in neuronal development
Description	Neuron development requires an elaborate network of endosomal transport pathways, defects in which underlie multiple neurological diseases. DENND10/FAM45A is an ancient protein that is important for endosomal homeostasis. However, the role of DENND10 in the nervous system remained unknown. Here, we impaired DENND10 expression in CAD neuronal cells by CRISPR editing. DENND10 deficiency resulted in shortened neurites, which was rescued by the re-expression of DENND10. Expression levels of neuronal specific cytoskeleton protein Tubb3 and tyrosine hydroxylase (Th) were also suppressed by DENND10 deletion. Here we utilized TMT-based quantitative mass spectrometry to study the proteomic landscape of DENND10-impaired CAD neurons.
HostingRepository	PRIDE
AnnounceDate	2025-03-06
AnnouncementXML	Submission_2025-03-06_07:01:53.206.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanling Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro; Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 06:21:40	ID requested
⏵ 1	2025-03-06 07:01:53	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: neuron, neurite outgrowth,DENND10, CAD

submitter keyword: neuron, neurite outgrowth,DENND10, CAD

Contact List

Yanling Zhang
contact affiliation	Soochow University
contact email	yanlzhan@suda.edu.cn
lab head
Yanling Zhang
contact affiliation	Soochow University
contact email	yanlzhan@suda.edu.cn
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD038623
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD038623

PRIDE project URI

Repository Record List

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