PXD038582-1

PXD038582 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) co-immunoprecipitated using a time course of infected buffalo sera
Description	Widespread Fasciola gigantica infection in buffaloes has caused great economic losses in buffalo farming. Studies on F. gigantica excretory and secretory products (FgESP) have highlighted their importance in F. gigantica parasitism and their potential in vaccine development. Identifying FgESP components involved in F. gigantica-buffalo interactions during different periods is important for developing effective strategies against fasciolosis. Buffaloes were assigned to non-infection (n = 3, as control group) and infection (n = 3) groups. The infection group was orally administrated 250 metacercariae. Sera were collected at 3, 10, and 16 weeks post-infection (wpi) for the non-infection group and at 0 (pre-infection), 1, 3, 6, 8, 10, 13, and 16 wpi for the infection group. FgESP components interacting with sera from the non-infection and infection groups assay were pulled down by co-IP and identified using LC-MS/MS. Interacting FgESP components in infection group were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway and gene ontology (GO) functional annotation to infer their potential functions. Proteins of FgESP components identified in the non-infection group at 3, 10, and 16 wpi accounted for 80.5%, 84.3%, and 82.1% of all proteins identified in these 3 time points, respectively, indicating surroundings did not affect buffalo immune response during maintenance. 490 proteins were identified in the infection group, of which 87 were consistently identified at 7 time points. Following GO analysis showed that most of these 87 proteins were in biological processes, while KEGG analysis showed they mainly functioned in metabolism and cellular processing, some of which were thought to functions throughout the infection process. The numbers of specific interactors identified for each week were 1 (n = 12), 3 (n = 5), 6 (n = 8), 8 (n = 15), 10 (n = 23), 13 (n = 22), and 16 (n = 14) wpi, some of which were thought to functions in specific infection precess. This study screened the antigenic targets in FgESP during a dense time course over a long period. These findings may enhance the understanding of molecular F. gigantica-buffalo interactions and help identify new potential vaccine and drug target candidates.
HostingRepository	PRIDE
AnnounceDate	2022-12-12
AnnouncementXML	Submission_2022-12-11_22:57:53.565.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	XinpingKong
SpeciesList	scientific name: Fasciola; NCBI TaxID: NCBITaxon:6191; scientific name: Fasciola gigantica; NCBI TaxID: 46835;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600+

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-06 07:15:43	ID requested
⏵ 1	2022-12-11 22:57:53	announced

Dataset with its publication pending

submitter keyword: Fasciola gigantica

co-immunoprecipitation

excretory and secretory products

interaction

LC-MS/MS

screening

WendaDi
contact affiliation	College of Animal Science and Technology, Guangxi University, Nanning,China
contact email	diwenda@gxu.edu.cn
lab head
XinpingKong
contact affiliation	Guangxi university
contact email	1609595283@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038582
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) co-immunoprecipitated using a time course of infected buffalo sera