PXD038575-2

PXD038575 is an original dataset announced via ProteomeXchange.

Title	Mammalian oocytes store proteins in cytoplasmic lattices
Description	Oocytes must accumulate and store maternal factors such as proteins during growth to sustain the first stages of embryonic development. How mammalian oocytes store maternal proteins is not understood. Here, we show that mouse and human oocytes store proteins on cytoplasmic lattices. With super-resolution light microscopy and electron tomography, we demonstrate that cytoplasmic lattices are twisted filament bundles formed by proteins of the subcortical maternal complex, filling the entire ooplasm. The lattices were associated with many proteins that have crucial functions during early embryo development, including proteins controlling genome methylation. Loss of cytoplasmic lattices prevented the accumulation of these proteins and resulted in early embryonic arrest. Thus, cytoplasmic lattices are important for storage of essential maternal proteins and the developmental success of early mammalian embryos.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:07:00.465.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luisa Welp
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-06 02:59:23	ID requested
1	2023-10-23 04:40:08	announced
⏵ 2	2024-10-22 06:07:00	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: DIA, Cytoplasmic lattices,Oocytes, LC-MS/MS

Melina Schuh
contact affiliation	Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
contact email	melina.schuh@mpinat.mpg.de
lab head
Luisa Welp
contact affiliation	Max-Planck-Institute for Biophysical Chemistry
contact email	lwelp@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038575

PRIDE project URI

• PRIDE
  1. PXD038575
     1. Label: PRIDE project
     2. Name: Mammalian oocytes store proteins in cytoplasmic lattices