PXD038561-1

PXD038561 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Substrate profiling of the metalloproteinase ovastacin – Implications for its physiological function in mammalian fertilization
Description	The metalloproteinase ovastacin is released by the mammalian oocyte upon fertilization and cleaves zona pellucida protein 2, a component of the surrounding extracellular matrix, at a distinct cleavage site. This limited proteolysis hardens the zona pellucida, abolishes sperm binding and thereby regulates fertility. Therefore, this process has to be tightly controlled by the plasma protein fetuin-B, an endogenous competitive inhibitor. At present, little is known about how the cleavage characteristics of ovastacin differ from closely related proteases. Physiological implications of ovastacin beyond ZP2 cleavage are still obscure. In this study, we employed N-terminal amine isotopic labeling of substrates (N-TAILS) contained in the secretome of mouse embryonic fibroblasts to elucidate the substrate specificity and the precise cleavage site specificity. Furthermore, we were able to unravel the physicochemical properties governing enzyme-substrate interactions. Eventually, we identified several potential physiological substrates with significance for mammalian fertilization. These results suggest that ovastacin might affect sperm interaction beyond zona pellucida hardening
HostingRepository	jPOST
AnnounceDate	2023-09-15
AnnouncementXML	Submission_2023-09-15_05:06:46.970.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ute Distler
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification; unknown modification
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-05 08:23:06	ID requested
⏵ 1	2023-09-15 05:06:47	announced

Publication List

Felten M, Distler U, von Wiegen N, Ł, ą, cki M, Behl C, Tenzer S, St, ö, cker W, K, ö, rschgen H, Substrate profiling of the metalloproteinase ovastacin uncovers specific enzyme-substrate interactions and discloses fertilization-relevant substrates. FEBS J, 291(1):114-131(2024) [pubmed]

Felten M, Distler U, von Wiegen N, Ł, ą, cki M, Behl C, Tenzer S, St, ö, cker W, K, ö, rschgen H, Substrate profiling of the metalloproteinase ovastacin uncovers specific enzyme-substrate interactions and discloses fertilization-relevant substrates. FEBS J, 291(1):114-131(2024) [pubmed]

Keyword List

submitter keyword: TAILS, MEF, Ovastacin

submitter keyword: TAILS, MEF, Ovastacin

Contact List

Hagen Körschgen
lab head
Ute Distler
contact affiliation	University Medical Center Mainz
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001944/