PXD038557-2

PXD038557 is an original dataset announced via ProteomeXchange.

Title	Brain heterotopia formation by ciliopathic breakdown of neuroepithelial and blood-cerebrospinal fluid barriers
Description	In this study, we found that ablation of genes encoding ciliary transport proteins such as intraflagellar transport homolog 88 (Ift88) and kinesin family member 3a (Kif3a) in cortical radial progenitors led to periventricular heterotopia during late mouse embryogenesis. Conditional mutation of primary cilia unexpectedly caused breakdown of both the neuroepithelial lining and the blood-choroid plexus barrier. Choroidal leakage was partially caused by enlargement of the choroid plexus in the cilia mutants. We found that the choroid plexus expressed platelet-derived growth factor A (Pdgf-A) and that Pdgf-A expression was ectopically increased in cilia-mutant embryos. Cortices obtained from embryos in utero electroporated with Pdgfa mimicked periventricular heterotopic nodules of the cilia mutant.
HostingRepository	PRIDE
AnnounceDate	2023-01-13
AnnouncementXML	Submission_2023-01-13_15:15:34.455.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JaemyungJang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-05 06:26:03	ID requested
1	2023-01-09 17:39:20	announced
⏵ 2	2023-01-13 15:15:34	announced	2023-01-13: Updated project metadata.
3	2023-11-14 08:58:36	announced	2023-11-14: Updated project metadata.

submitter keyword: cerebrospinal fluid,LC-MSMS

YoungshikChoe1
contact affiliation	1Korea Brain Research Institute
contact email	dallarae@kbri.re.kr
lab head
JaemyungJang
contact affiliation	Korea Brain Research Institute
contact email	piloter2@kbri.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD038557

PRIDE project URI

• PRIDE
  1. PXD038557
     1. Label: PRIDE project
     2. Name: Brain heterotopia formation by ciliopathic breakdown of neuroepithelial and blood-cerebrospinal fluid barriers