PXD038549-3

PXD038549 is an original dataset announced via ProteomeXchange.

Title	Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton
Description	Coastal upwelling regions are among the most productive marine ecosystems but may be threatened by amplified ocean acidification. Increased acidification is hypothesized to reduce iron bioavailability for phytoplankton thereby expanding iron limitation and impacting primary production. Here we show from community to molecular levels that phytoplankton in an upwelling region respond to short-term acidification exposure with iron uptake pathways and strategies that reduce cellular iron demand. A combined physiological and multi-omics approach was applied to trace metal clean incubations that introduced 1200 ppm CO2 for up to four days. Although variable, molecular-level responses indicate a prioritization of iron uptake pathways that are less hindered by acidification and reductions in iron utilization. Growth, nutrient uptake, and community compositions remained largely unaffected suggesting that these mechanisms may confer short-term resistance to acidification; however, we speculate that cellular iron demand is only temporarily satisfied, and longer-term acidification exposure without increased iron inputs may result in increased iron stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:14:06.820.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038549
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Loay Jabre
SpeciesList	scientific name: marine plankton environmental sample; NCBI TaxID: 632957;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-05 03:05:51	ID requested
1	2023-11-14 00:09:41	announced
2	2023-11-14 07:50:32	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:14:07	announced	2024-10-22: Updated project metadata.

10.6019/PXD038549;

10.1038/S41467-023-42949-1;

submitter keyword: marine microbes,metaproteomics, phytoplankton, ocean acidification

Erin M.
contact affiliation	Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada
contact email	erin.bertrand@dal.ca
lab head
Loay Jabre
contact affiliation	Dalhousie University
contact email	loay.jabre@dal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD038549

PRIDE project URI

• PRIDE
  1. PXD038549
     1. Label: PRIDE project
     2. Name: Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton