PXD038528-1

PXD038528 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of extracellular vesicles enriched from plasma of patients with lung adenocarcinoma and malignant melanoma.
Description	We present an optimized HiRIEF LC-MS workflow to perform an in-depth proteome profiling of plasma derived extracellular vesicles (pEVs). Here, we have applied the HiRIEF pre-fractionation and tandem mass tags (TMT)-based peptide quantification to generate pEV proteomes of 6 malignant melanoma (MM) and 6 lung adenocarcinoma (LUAD) patients. We have detected traditional EV-marker proteins, tetraspanins, ESCRTs, and several other proteins associated to EV cargo selection, trafficking/sorting, and exosome biogenesis. Importantly, we also detected many LUAD and MM related proteins in the cancer pEVs. Our advanced proteomics workflow exhibits high pEV proteome coverage and the ability to detect potential disease-specific markers in pEVs enriched from clinical plasma samples.
HostingRepository	PRIDE
AnnounceDate	2024-08-15
AnnouncementXML	Submission_2024-08-15_03:00:57.870.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nidhi Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-03 17:34:48	ID requested
⏵ 1	2024-08-15 03:00:58	announced
2	2024-10-22 06:55:07	announced	2024-10-22: Updated project metadata.

10.1021/ACS.JPROTEOME.4C00490;

submitter keyword: cancer, lung adenocarcinoma, proteomics,extracellular vesicles, mass spectrometry, plasma, liquid biopsy, HiRIEF, malignant melanoma

Janne Lehtiö
contact affiliation	Department of Oncology Pathology, Karolinska Institute, Solna, Sweden
contact email	janne.lehtio@ki.se
lab head
Nidhi Sharma
contact affiliation	Karolinska Institute
contact email	nidhi.sharma@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD038528

PRIDE project URI

• PRIDE
  1. PXD038528
     1. Label: PRIDE project
     2. Name: Proteome profiling of extracellular vesicles enriched from plasma of patients with lung adenocarcinoma and malignant melanoma.