PXD038436-1

PXD038436 is an original dataset announced via ProteomeXchange.

Title	Proteomics profiling reveals regulation of immune response to Salmonella enterica serovar Typhimurium infection in mice
Description	Regulation of the immune response to Salmonella enterica serovar Typhimurium (S. Typhimurium) infection is a complex process, influenced by the interaction between genetic and environmental factors. Different inbred strains of mice exhibit distinct levels of resistance to S. Typhimurium infection, ranging from susceptible (e.g., C57BL/6J) to resistant (e.g., DBA/2J) strains. However, the underlying molecular mechanisms contributing to the host response remain elusive. In this study, we present a comprehensive proteomics profiling of spleen tissues from C57BL/6J and DBA/2J strains with different doses of S. Typhimurium infection by tandem tag mass coupled with two-dimensional liquid chromatography-tandem mass spectrometry (TMT-LC/LC-MS/MS). We identified and quantified 3,986 proteins, resulting in 475 differentially expressed proteins (DEPs) between C57BL/6J and DBA/2J strains. Functional enrichment analysis unveiled that the mechanism of innate immune responses to S. Typhimurium infection could be associated with several signaling pathways, including the interferon signaling pathway. We experimentally validated the roles of interferon signaling pathway in innate immune response to S. Typhimurium infection using IFN-γ neutralization assay. We further illustrated the roles of macrophage cells and pro-inflammatory cytokines in the mechanisms underlying the resistance to S. Typhimurium using qRT-PCR. Taken together, our results provide new insights into the genetic regulation of the immune response to S. Typhimurium infection in mice and might provide potential protein targets for controlling the infection.
HostingRepository	PRIDE
AnnounceDate	2022-12-01
AnnouncementXML	Submission_2022-12-01_12:05:12.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LingLi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-27 00:08:19	ID requested
⏵ 1	2022-12-01 12:05:12	announced
2	2023-11-14 08:58:51	announced	2023-11-14: Updated project metadata.

submitter keyword: Proteome

Proteomics

Mass Spectrometry

Immune Response

Genetic Regulation

Salmonella

S. Typhimurium

Mouse

XushengWang
contact affiliation	Department of Biology, University of North Dakota, Grand Forks, ND 58202, USA
contact email	xusheng.wang@UND.edu
lab head
LingLi
contact affiliation	University of North Dakota
contact email	ling.li.1@und.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038436
     1. Label: PRIDE project
     2. Name: Proteomics profiling reveals regulation of immune response to Salmonella enterica serovar Typhimurium infection in mice