PXD038404-3

PXD038404 is an original dataset announced via ProteomeXchange.

Title	Male mammalian meiosis and spermiogenesis is critically dependent on the shared functions of the katanin proteins KATNA1 and KATNAL1
Description	Katanin microtubule severing enzymes are potent M-phase regulators across eukaryotes. The requirement of katanin enzymatic A-subunits in male mammalian meiosis, however, remains untested. Here, using multiple single and double gene knockout mice, we reveal katanin A-subunits are essential for male meiosis, and in doing so demonstrate functional compensation exists among mammalian katanin A-subunit paralogs. We show KATNA1 and KATNAL1 collectively regulate the male meiotic spindle, germ cell microtubule bulk, in addition to diverse spermatid remodelling events, including Golgi organisation, pro-acrosomal vesicle trafficking and manchette formation. We also define KATNAL1-specific roles in sperm flagella development, manchette regulation, and sperm-epithelial disengagement. Finally, using immunoprecipitation-mass spectrometry approaches we define the KATNA1, KATNAL1, and KATNB1 mammalian testis interactome, which includes a network of cytoskeletal and vesicle trafficking proteins. Collectively, we reveal the presence of multiple katanin A-subunit paralogs in mammalian spermatogenesis allows for both ‘customized cutting’ via neofunctionalization and protective buffering via gene redundancy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:39.407.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Steele
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 04:14:05	ID requested
1	2023-10-30 17:12:54	announced
2	2023-11-14 08:31:30	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:39	announced	2024-10-22: Updated project metadata.

10.1242/DEV.201956;

submitter keyword: axoneme, acrosome, manchette, vesicle transport, spermiation, microtubule severing,Meiotic spindle, spermatogenesis, HTCA, male fertility

Jessica Dunleavy
contact affiliation	School of BioSciences and Bio21 Institute, Faculty of Science, The University of Melbourne, VIC, 3010, Australia.
contact email	Jessica.dunleavy@unimelb.edu.au
lab head
Joel Steele
contact affiliation	Monash
contact email	joel.steele@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038404

PRIDE project URI

• PRIDE
  1. PXD038404
     1. Label: PRIDE project
     2. Name: Male mammalian meiosis and spermiogenesis is critically dependent on the shared functions of the katanin proteins KATNA1 and KATNAL1