PXD038402-3

PXD038402 is an original dataset announced via ProteomeXchange.

Title	The Respiratory Syncytial Virus M2-2 protein is targeted for proteasome degradation and inhibits translation and stress granules assembly
Description	The M2-2 protein from the respiratory syncytial virus (RSV) is a 10 kDa protein expressed by the second ORF of the viral gene M2. During infection, M2-2 has been described as the polymerase cofactor responsible for promoting genome replication. This function was first inferred by infection with a mutant virus lacking the M2-2 ORF, in which viral genome presented delayed accumulation in comparison to wild-type virus. In accordance with this phenotype, it has been recently shown that M2-2 promotes changes in interactions between the polymerase and other viral proteins at early stages of infection. Despite its well explored role in the regulation of the polymerase activity, little has been made to investigate the relationship of M2-2 with cellular proteins. In fact, previous reports showed poor recruitment of M2-2 to viral structures, with the protein being mainly localized to the nucleus and cytoplasmic granules. To unravel which other functions M2-2 exerts during infection, we expressed the protein in HEK293T cells and performed proteomic analysis of co-immunoprecipitated partners, identifying enrichment of proteins involved with regulation of translation, protein folding and mRNA splicing. In approaches based on these data, we found that M2-2 expression downregulates eiF2α phosphorylation and inhibits stress granules assembly under arsenite induction. In addition, we also verified that M2-2 inhibits translation initiation, and is targeted for proteasome degradation, being localized to granules composed by defective ribosomal products at the cytoplasm. These results suggest that besides its functions in the regulation of genome replication, M2-2 may exert additional functions to contribute to successful RSV infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:08:37.348.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronica Santiago
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 04:13:11	ID requested
1	2023-10-24 11:05:13	announced
2	2023-11-14 09:07:09	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:08:38	announced	2024-10-22: Updated project metadata.

10.1371/journal.pone.0289100;

Scudero OB, Santiago VF, Palmisano G, Simabuco FM, Ventura AM, The respiratory syncytial virus M2-2 protein is targeted for proteasome degradation and inhibits translation and stress granules assembly. PLoS One, 18(7):e0289100(2023) [pubmed]

submitter keyword: RSV

syncytial virus

M2-2

translation

stress granules

eiF2α

proteasome

DRiPs

puromycin.

Armando Morais Ventura
contact affiliation	Laboratory of RNA Virus Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
contact email	amventur@icb.usp.br
lab head
Veronica Santiago
contact affiliation	University of Southampton
contact email	veronicafeijoli@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038402
     1. Label: PRIDE project
     2. Name: The Respiratory Syncytial Virus M2-2 protein is targeted for proteasome degradation and inhibits translation and stress granules assembly