PXD038389-3

PXD038389 is an original dataset announced via ProteomeXchange.

Title	Differential modulation of the phosphoproteome by the MAP Kinases isoforms p38α and p38β
Description	The stress-activated p38 mitogen activated protein kinases (MAPK) mediate responses to osmotic shock, radiation, immune stimuli, inflammatory cues, and many other stresses. These kinases are activated rapidly, within seconds of stress induction, yet, their continuous activation, as commonly happens in inflammations and cancer, induce different signaling cascades than transient activation. This study aimed to follow the specific signaling cascades induced by two of these p38 MAPKs activated by strong and rapid stress, or by continuous (chronic) activations. The analysis was based on large-scale proteomics and phosphoproteomics analyses using SILAC labeling of mouse embryonic fibroblasts (MEF) deficient in each of these p38 kinases, expressing constitutively expressed wildtype p38α or p38β, or their intrinsically active variants. In addition, the effects of anisomycin, inducing strong and rapid stress response, were followed in wildtype MEF or in MEF knocked-out for these p38α or p38β. The signaling events, induced the each p38 during the chronic responses, indicated adaptations of the cells expressing the intrinsically active p38 mutants. The continuous activities of the individual p38 induced feedback loops that inactivated the other p38s. Furthermore, a number of new phosphorylation sites on proteins relevant to stress responses and cancer, such as p53 and p27, were revealed in this study.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:06.897.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dganit Melamed Kadosh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 03:57:39	ID requested
1	2023-10-24 15:17:40	announced
2	2023-11-14 09:12:44	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:11	announced	2024-10-22: Updated project metadata.

Melamed Kadosh D, Beenstock J, Engelberg D, Admon A, . Int J Mol Sci, 24(15):(2023) [pubmed]

10.3390/ijms241512442;

submitter keyword: phosphoproteome, SILAC, stress response,p38 MAPK, signaling

Arie Admon
contact affiliation	Faculty of Biology, Technion Israel institute of technology, Haifa, Israel
contact email	admon@technion.ac.il
lab head
Dganit Melamed Kadosh
contact affiliation	Technion
contact email	sdganit@tx.technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038389
     1. Label: PRIDE project
     2. Name: Differential modulation of the phosphoproteome by the MAP Kinases isoforms p38α and p38β