PXD038378 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeting an RNA polymerase II-associated PHF5A protein subcomplex with a KMT2A-WDR5 inhibitor impairs self-renewal and tumorigenicity of pancreatic cancer stem cells |
Description | Pancreatic cancer (PC) remains one of the most aggressive and life-threatening malignancies known for its notorious resistance to chemotherapy. This is increasingly ascribed to the subpopulation of undifferentiated cells, known as pancreatic cancer stem cells (PCSCs), which are evolutionary fitter than other tumor cells to evade the cytotoxic effects of chemotherapy. Those cells are crucial for tumor relapse as they possess ‘stem cell-like’ features of self-renewal and differentiation. However, what molecular mechanisms maintain the unique characteristics of PCSCs are poorly understood. Here, we identified an RNA polymerase II-associated PHF5A-PHF14-HMG20A-RAI1-KMT2A transcriptional subcomplex, which regulates the stemness characteristics and tumorigenicity of PCSCs through epigenetic control of gene expression. Targeting the protein subcomplex with a KMT2A-WDR5 inhibitor attenuated the self-renewal and in vivo tumorigenicity of PCSCs, thus offering a novel anti-PCSCs targeting strategy for enhancing the efficiency of chemotherapy which is likely to translate into durable clinical responses in PC patients. |
HostingRepository | PRIDE |
AnnounceDate | 2023-08-23 |
AnnouncementXML | Submission_2023-08-23_04:06:44.991.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | SiweiDeng |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Dionex instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-26 03:46:39 | ID requested | |
⏵ 1 | 2023-08-23 04:06:45 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: epigenetics, cancer stem cells,Pancreatic cancer, PHF5A complex, KMT2A-WDR5 inhibitor |
Contact List
SiimPauklin |
contact affiliation | Botnar Research Centre, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, UK |
contact email | siim.pauklin@ndorms.ox.ac.uk |
lab head | |
SiweiDeng |
contact affiliation | Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford |
contact email | siwei.deng@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038378
- Label: PRIDE project
- Name: Targeting an RNA polymerase II-associated PHF5A protein subcomplex with a KMT2A-WDR5 inhibitor impairs self-renewal and tumorigenicity of pancreatic cancer stem cells